Chronic effects of soft drink consumption on the health state of Wistar rats: A biochemical, genetic and histopathological study
- Authors:
- Published online on: April 27, 2016 https://doi.org/10.3892/mmr.2016.5199
- Pages: 5109-5117
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Copyright: © Alkhedaide et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Soft drinks consumption (SDC) has increased worldwide in the past two-three decades (1). Their effects on health are unclear, although epidemiological studies pointed toward its associations with obesity, kidney, liver diseases and osteoporosis (2,3). Soft drinks contain predominantly water, phosphoric acid, caffeine, sugar in the form of sucrose and other chemicals in the form of preservatives, colorings and flavors (2). The rate of SDC is alarming, particularly in the affluent countries (4). Numerous various health problems are associated with regular consumption of soft drinks (2). It has been shown that ~25 separate harmful effects are associated with SDC (2,3). Contents of soft drinks have a bad effect on human health (4). Caffeine in carbonated beverages is deliberately added to make individuals addicted, and is readily absorbed rapidly compared with any other drink (5).
A correlation exists between SDC and the incidence of multiple diseases, including obesity, dental/bone problems, diabetes mellitus and cardiovascular disease (6–9). Additionally, SDC is notbaly associated with kidney health and a high risk of kidney stone formation (10,11). SDC causes bone fracture, disruption in bone formation, affects serum or urinary calcium metabolism markers and hypocalcemia, both in clinical and experimental settings (4,12–16). However, no significant association between SDC and the reduction of bone mineral density were reported in previous studies (17,18). Therefore, the effect of SDC on bone, liver and renal health remains unknown and proper evidence is still required. The liver is the predominant organ in the human body that serves an essential role in the metabolism of food and drugs, and any alteration in its function induces deleterious effects. Additionally, the kidney is an organ whose functions in either the removal of metabolic waste products from the blood and water, or the regulation of electrolytes. The majority of published papers focused on biochemical alterations induced by soft drinks on the serum levels of hepatic and renal biomarkers.
In Saudi Arabia and the Middle East, individuals are used to drinking soft drinks on average 3 times per day with meals. Therefore, the present study aimed to examine the effect of chronic SDC (Coca-cola, Pepsi and 7-up) on oxidative stress biomarkers, histopathology of bone, liver and kidney and the expression of certain genes associated with liver and kidney functions, in order to outline its effect on the health of Wistar rats.
Materials and methods
Chemicals and kits
Ethidium bromide for agarose preparation and agarose were purchased from Sigma-Aldrich (St. Louis, MO, USA). A total of 40 male Wistar rats were purchased from King Abdel-Aziz University, King Fahd Center for Scientific Research (Jeddah, Saudi Arabia). Catalase, malondialdehyde (MDA), glutathione peroxidase (GSH-Px), glutathione reductase (GR), creatinine and urea kits were purchased from Bio Diagnostic Co. (Dokki, Egypt). Coca-Cola, Pepsicola and 7-Up were purchased from Taif markets in Saudi Arabia. The 100 bp DNA ladder marker was purchased from Fermentas (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Oligo dT primer and Qiazol reagent were purchased from Qiagne (Valencia, CA, USA).
Experimental animals, design and sampling
The present study was approved by the Ethics Committee for the College of Applied Medical Sciences (Taif University; no. 3792/34/1). A total of 40 male Wistar rats (12-weeks-old; weight, 200–280 g). The rats were monitored daily and were maintained under observation for 1 week for complete acclimatization. The rats were maintained at 12 h light/dark cycles and received free access to food and water for the first week. The rats were then divided into four groups: i) Control without any treatment; ii) Coca-cola group; iii) Pepsi group; iv) 7-Up group. The rats in groups 2–4 received free access to the respective soft drinks for three consecutive months. At the end of experiment, all rats were anesthetized by diethyl ether inhalation. Blood and tissues were collected from anesthetized rats in sterilized vacutainer tubes. The serum was extracted following centrifugation of clotted blood for 15 min at 3,000 × g and was maintained at −20°C until use. For gene expression analysis, hepatic and renal tissues were maintained at −80°C in Qiazol reagent for RNA extraction. For histopathological examination, sections from the bone, liver and kidney were immersed overnight in neutral buffered formalin (NBF; 10%) at room temperature.
Serum biochemical assays
MDA, GSH-Px, GSH-reductase (GSH-R), catalase, glucose, alkaline phosphatase (ALP), urea, bilirubin, creatinine, alanine transaminase (ALT), uric acid, aspartate transaminase (AST), creatine kinase (CK) and CKM were assayed spectrophotometrically using commercial kits purchased from Bio Diagnostic Co., according to the manufacturer's protocol. The levels of osteocalcin levels were assayed using enzyme-linked immunosorbent assay kits purchased from MyBioSource, LLC, San Diego, CA, USA). Various minerals, triglycerides (TG), cholesterol and high-dentisty lipoproteins (HDL) were assayed spectrophotometrically using kits imported from HUMAN Gesellschaft für Biochemica und Diagnostica mbH (Wiesbaden, Germany), according to the manufacturer's protocol. Serum lipase was measured using a lipase activity assay kit imported from Abcam (Cambridge, MA, USA).
cDNA preparation, synthesis and gene expression assessments
The total RNA from tissue samples was extracted, as previously described (19). The integrity of RNA was visualized and confirmed following running on a denatured agarose gel (1.5%) stained with ethidium bromide. Oligo dT primer (0.5 ng) was added to 2 µg total RNA to induce denaturation and was subsequently used for cDNA synthesis (19). For semi-quantitative gene expression analysis, specific primers were designed for certain genes, as illustrated in Table I, using Oligo 4 computer program (version 7; Molecular Biology Insights, Inc., Colorado Springs, CO, USA) and synthesized by (Macrogen Company, Geumcheongu. Korea). PCR was performed in a total volume of 25 µl (1 µl cDNA, 1 µl 10 pM each primer, 12.5 µl PCR master mix and 9.5 µl sterilized, deionized water). A Bio-Rad T100™ Thermal Cycle machine (Bio-Rad Laboratories, Inc., Hercules, CA, USA) was used for PCR, which was performed at 95°C for 4 min, followed by 33 cycles of denaturation at 95°C for 50 sec, annealing as shown in Table I and extension at 72°C for 1 minute, with an additional final extension at 72°C for 5 min. The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as internal standard and as a reference gene. The PCR products were electrophoresed in a 1.5% agarose gel, followed by staining with ethidium bromide in Tris-Borate-EDTA buffer. The bands were visualized using an InGenius 3.0 gel documentation system (Syngene, Frederick, MD, USA) under UV light.
Histopathology of bone, liver and kidney
Tissues from the bone, liver and kidney were dissected following diethyl ether inhalation and the sacrificing of the rats. The samples were immersed and fixed overnight in a 10% NBF solution. Bone, liver and kidney tissues were processed, washed and preserved in ethanol (70%). The samples were subsequently dehydrated in ethanol solution in ascending grades, cleared with xylene, paraffin wax embedded, casted and cut into 5 µm sections using microtome (Leica Microsystems, Inc., Buffalo Grove, IL, USA). Mayer's hematoxylin and eosin, and Masson's trichrome staining were used to stain the slides with the fixed tissue samples (20). The slides with tissue were examined using a Wolfe S9-0982 microscope (Carolina Biological Supply Company, Burlington, NC, USA) and images were captured using a Canon Power-Shot SX500 IS digital camera.
Statistical analysis
The data are presented as the mean ± standard error of the mean. Analysis of variance was used to analyze data together with post hoc descriptive tests using SPSS 11.5 for Windows (SPSS, Inc., Chicago, IL, USA). P<0.05 was considered to indicate a statistically significant difference. The intensities of the bands were quantified densitometrically using Image J software (version 1.47) and were matched compared to the control group and expressed as a percent (http://imagej.en.softonic.com/).
Results
Effect of SDC for 3 months on the serum levels of MDA, GSH-Px, catalase and GSH-R in Wistar rats
SDC for 3 months revealed an increase in MDA levels (Table II) with a highly significant effect on Pepsi-administered rats compared with the control rats. By contrast, the antioxidant activity of GSH-Px, catalase and GSH-R in the rats were decreased significantly in all SDC groups (Table II).
 | Table IISerum changes in MDA, GSH-Px, GSH-R and catalase levels in rats following chronic consumption of soft drinks. |
Effect of SDC for 3 months on the blood levels of glucose, hepatic and renal biomarkers in Wistar rats
SDC for 3 months, significantly increased the serum levels of glucose and moderately increased uric acid and bilirubin in the Coca-Cola, Pepsi and 7-up groups compared with the control group (Table III).
| Table IIISerum changes in glucose, urea, uric acid, creatinine, and total and direct bilrubin levels in rats following chronic consumption of soft drinks. |
Effect of SDC for 3 months on serum levels of hepatic biomarkers in Wistar rats
SDC for 3 months significantly increased the levels of liver biomarkers, with a more marked effect on AST and a moderate effect on ALT. Additionally, ALP increased in all groups compared with the control rats (Table IV).
| Table IVSerum changes in AST, ALT and ALP levels in rats following chronic consumption of soft drinks. |
Effect of SDC for 3 months on the serum levels of minerals and osteocalcin in Wistar rats
SDC decreased both calcium and iron levels in the rats. By contrast soft drinks increased serum phosphorous levels. In addition, soft drinks revealed no effect sodium and chloride levels. Notably, soft drinks increased the levels of osteocalcin (Table V).
Effect of SDC for 3 months on the serum levels of lipase and lipid profiles in Wistar rats
SDC increased serum lipase levels, which resulted in a decrease in cholesterol and TG levels in the SDC groups compared with the control (Table VI). By contrast, HDL was decreased in the Coca-Cola and Pepsi groups compared with the control group; however, 7-Up caused no effect.
| Table VISerum changes in lipid profiles and lipase levels in rats following chronic consumption of soft drinks. |
Effect of SDC for 3 months on the mRNA expression levels of GST and SOD in the liver of Wistar rats
SDC for 3 months downregulated the gene expression levels of GST and SOD (Fig. 1). The expression percentage of GST was ~80, 60 and 40% decreased in Coca-Cola, Pepsi and 7-Up groups, respectively. All groups exhibited a slight downregulation in the mRNA expression of SOD in hepatic tissue at a percentage of 30, 18 and 15%, respectively, compared with the control rats.
Effect of SDC for 3 months on the mRNA expression levels of α1-AGP and α2-MG in the liver of Wistar rats
SDC significantly upregulated the mRNA expression of α1-AGP in the liver tissues (Fig. 2). The percentage of upregulation in the expression of α1-AGP was 66, 70 and 75% for Coca-Cola, Pepsi and 7-Up, respectively. By contrast, the expression of α2-MG was downregulated by Coca-Cola (45%) and moderately upregulated in the Pepsi and 7-Up groups by 15 and 20%, respectively, compared with the control rat (Fig. 2).
Effect of SDC for 3 months on the mRNA expression levels of genes associated with renal function biomarkers and stability in Wistar rats
Next, the present study tested the effect of SDC on the mRNA expression levels of certain genes associated with normal kidney function and stability. The expression of desmin was upregulated in the Coca-Cola-, Pepsi- and 7-Up-administered rats at a percentage of 75, 50 and 60%, respectively (Fig. 3). Interleukin (IL)-1β was only increased in the Pepsi and 7-Up groups by 30 and 50%, respectively. The expression of homooxygenase (HO)-1 was only increased in the Coca-Cola and 7-Up groups by 90 and 25%, respectively. The present study also examined the genes associated with blood pressure, including angiotensinogen (AT) and its receptor (AT-R). The expression of AT was upregulated in the Coca-Cola group (55% increase compared with the control). The expression of AT-R was also upregulated by 64 and 10% for Cola and Pepsi, respectively, compared with the control and 7-Up groups (Fig. 3).
Effect of SDC for 3 months on bone, liver and kidney histopathology
The bone consisted of numerous bony lamellae, and bone marrow extended between these lamella. The blood progenitor cells occupied the architecture of the bone marrow. The structure unit of the bone consisted of osteocytes arranged around the haversian canal (Fig. 4A). In the Coca-Cola and Pepsi groups, the bone lamella exhibited dark acidophilic areas in the bone architecture due to calcium withdrawal, while the other parts exhibited faint basophilic patches (Fig. 4B and C). In the 7-Up group, the bone lamella exhibited little histological changes in the form of small patches of faint basophilic areas (Fig. 4D).
The liver consisted of a central vein, which was surrounded by numerous hepatic cords. The hepatic cords were synthesized from polygonal hepatic cells with acidophilic cytoplasm and basophilic nuclei, which were centrally located. Von Kupffer cells were located between the hepatic cords (Fig. 5A). In the Coca-Cola group, the liver exhibited activation of Von Kupffer cells and congestion in the blood vessels (Fig. 5B). The liver of the Pepsi group exhibited congestion in blood vessels (Fig. 5C). In the 7-up group, the liver exhibited hydropic degeneration, in addition to congestion of the blood vessels (Fig. 5D).
The kidney of the control group consisted of renal corpuscle, surrounded by proximal and distal convoluted tubules (Fig. 6A). In all soft drink groups, the renal tissues exhibited mild congestion in the renal blood vessels (Fig. 6B–D).
Discussion
The results of the present study confirmed that chronic SDC induced oxidative stress, metabolic alterations and changes in gene expression in Wistar rats. Oxidative stress has been associated with the etiology and pathogenesis of various chronic diseases and serves a vital role in the aging process (21). High levels of free radicals or reactive oxygen species (ROS) can cause direct damage to lipids inside cells and induce peroxidation (21). The primary sources of endogenous ROS production are the mitochondria, plasma membrane, endoplasmic reticulum and peroxisomes through a variety of mechanisms, including enzymatic reactions and/or auto-oxidation of several compounds, including catecholamines and hydroquinone (22). Chronic SDC-induced oxidative stress, which may cause hepatic toxicity, is indicated by the increase in MDA and the decrease in GSH-Px, GSH-R and catalase levels, as well as the decrease in the mRNA expression levels of GST and SOD. Soft drinks are the predominant source of sugar worldwide and are associated with obesity in children and adolescents. Soft drinks favor the incidence of insulin resistance and inflammation, and other diseases, including obesity, type-2 diabetes, dental caries, osteoporosis and low nutrient level (23–25).
The present study hypothesized that SDC is associated with lower bone mineral density, as indicated by the disturbance in minerals levels that is likely due to its caffeine contents, which is a predisposing factor for osteoporosis incidence (5,26,27). In addition, phosphoric acid interferes with absorption of calcium and increases the loss of calcium (5). Additionally, high fructose may negatively affect bone stability (28). The increase in glucose reported in the present results is due to presence of caffeine in soft drinks. Caffeine causes the release of adrenaline and the increase in blood glucose to counteract the requirement for energy during emergency (3,5). SDC caused liver injuries that are followed by the increase in the activities of GPT and GOT (29). Alkaline phosphatase increase is considered as a bone remodeling marker. The increase in AP is due to the presence of caffeine in soft drinks (30). Caffeine affects the biosynthesis of AP and inhibits the formation of a competent extracellular matrix, essential for bone remodling (30,31). Additionally, the increase in osteocalcin that serves a role in mineralization of bone and calcium homeostasis cannot be neglected (32). It has been previously shown that heavy SDC is associated with hypocalcemia, and alterations in liver enzymes and histology in non-alcoholic fatty liver disease, both in clinical and experimental settings (5,12,13,33,34).
The explanation of mineral metabolism disorders is due to high phosphate intake as exogenous phosphate loads inhibits renal 1α-hydroxylase and reduces vitamin D biosynthesis (35). Therefore, the increase in phosphate hinders both intestinal absorption and renal calcium re-absorption causing hypocalcemia (36). Consequently, hyperparathyroidism is developed, however, this is not enough to prevent sustained hypocalcemia (36). This explains why heavy SDC is characterized by hypocalcemia, hyperparathyroidism and reduced 1α-25-dihy droxyvitamin D level (5,12). In parallel, SDC decreased iron absorption in the intestine, as explained in the present and a previous study (37). In addition, acidemia occurred when SDC increases bone resorption and calcium mobilization, and reduces 1α-hydroxylase activity and vitamin D production in kidney (38–40).
α1-AGP is a protein of acute phase stage, synthesized predominantly in hepatic cells and is regulated by pregnancy, drugs and certain diseases, and is increased during inflammation (41). The present study reported that SDC increased the mRNA expression of α1-AGP, as a result of hepatic cells injury. Additionally, α-2MG is slightly decreased in response to Coca-Cola and increased in response to Pepsi and 7-Up. α-2MG upregulation is probably a reversed mechanism to prohibit plasmin biosynthesis and to facilitate the transport of growth factors, which assist in the regeneration of hepatocytes due to hepatic congestion reported in histopathological findings (42). Renal podocytes are essential components of the kidney, which maintain normal glomerular structure. Podocytes regulate filtration flow through the intracellular spaces, and they are situated at the basement of glomeruli and act as ultrafilteration barrier (43,44). Focal segmental glomerulosclerosis and chronic renal diseases are caused by podocyte injury (45).
SDC upregulated the expression levels of desmin and HO-1 and angiotensinogen. Desmin upregulation is indicative for inflammation in the intrafilament proteins (46). HO-1 assists in the oxidation of heme producing active molecules (carbon monoxide, biliverdin and ferrous ion) that serve as a second messenger to control various cellular functions (47). Upregulation of HO-1 causes inhibition of either effectors of immune functions or adaptive response to various body injuries (48). The upregulation in the expression of IL-1β is proceeded by an increase in the expression of HO-1. Development of hypertension, renal oxidative stress and tissue injury are due to an increase in AGT biosynthesis, secretion and excretion (49). Of interest, the changes reported in the expression levels of AT-1 and AGT were due to changes in oxidative stress and renal injuries resulting from SDC, as confirmed by renal congestion. Bone is the most examined organ affected histopathologically due to minerals metabolism disorder, as reported in the present and other previous studies (16,18,26,27). The kidney and liver histopathological alterations reported are moderate degenerative changes, which resulted in the changes in gene expression of both the kidney and liver. Taken together, these data confirmed that SDC induced biochemical and genetic alterations in examined organs, although less histopathological changes reported in the bone, liver and kidney.
Acknowledgments
The present study was supported by a grant in aid for Mohamed Mohamed Soliman of the Deans of Scientific Research Affairs, Taif University, Saudi Arabia (no. 3792–1436).
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