Open Access

MicroRNA‑31 functions as an oncogenic microRNA in cutaneous squamous cell carcinoma cells by targeting RhoTBT1

  • Authors:
    • Nengxing Lin
    • Yu Zhou
    • Xin Lian
    • Yating Tu
  • View Affiliations

  • Published online on: Monday, January 2, 2017
  • Pages:1078-1082 DOI: 10.3892/ol.2017.5554
0

Abstract

Cutaneous squamous cell carcinoma (cSCC) is a malignancy of epidermal keratinocytes that is responsible for ~20% of annual skin cancer‑associated mortalities. Accumulating evidence demonstrates that the dysregulation of micro (mi)RNAs serves a significant role in the tumorigenesis and progression of human cSCC. MicroRNA‑31 (miR‑31) is upregulated in cSCC and is involved in cSCC development. However, the underlying mechanism remains unclear. The present study demonstrated that miR‑31 is upregulated in the cSCC cell line, A‑431, and that miR‑31 expression contributes to the cell proliferation and invasion of cSCC. In addition, bioinformatics combined with dual luciferase reporter analysis was applied to determine that the tumor suppressor RhoTBT1 was a direct target of miR‑31. In addition, miR‑31 mimics reduced RhoBTB1 expression in A‑431 cells. The results of MTT and Transwell assays demonstrated that knockdown of RhoBTB1 by short interfering RNA induced cell proliferation and invasion in A‑431 cells. These results indicated that suppression of RhoBTB1 may be involved in cSCC tumorigenesis, which was directly affected by miR‑31. In conclusion, the present study provides evidence that miR‑31 acts as an oncogene through direct repression of RhoTBT1 expression in cSCC cancer, suggesting a potential application of miR‑31 in prognosis prediction and its therapeutic application in cSCC.

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March 2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

2015 Impact Factor: 1.482
Ranked #97/211 Oncology
(total number of cites)

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APA
Lin, N., Zhou, Y., Lian, X., & Tu, Y. (2017). MicroRNA‑31 functions as an oncogenic microRNA in cutaneous squamous cell carcinoma cells by targeting RhoTBT1. Oncology Letters, 13, 1078-1082. http://dx.doi.org/10.3892/ol.2017.5554
MLA
Lin, N., Zhou, Y., Lian, X., Tu, Y."MicroRNA‑31 functions as an oncogenic microRNA in cutaneous squamous cell carcinoma cells by targeting RhoTBT1". Oncology Letters 13.3 (2017): 1078-1082.
Chicago
Lin, N., Zhou, Y., Lian, X., Tu, Y."MicroRNA‑31 functions as an oncogenic microRNA in cutaneous squamous cell carcinoma cells by targeting RhoTBT1". Oncology Letters 13, no. 3 (2017): 1078-1082. http://dx.doi.org/10.3892/ol.2017.5554