Open Access

Extracellular HSP70/HSP70‑PCs regulate hepatocarcinoma cell migration and invasion via RhoA

  • Authors:
    • Zhe Yi
    • Yan Li
    • Dan Liu
    • Jingang Liu
    • Hangyu Li
  • View Affiliations

  • Published online on: Friday, December 30, 2016
  • Pages:1095-1100 DOI: 10.3892/ol.2016.5551
0

Abstract

The effects of heat shock protein 70 (HSP70)/HSP70‑peptide complexes (HSP70‑PCs) on the invasion and metastasis ability of hepatocellular carcinoma (HCC) Huh‑7 cells were investigated. Wound healing assay revealed that cells treated with HSP70/HSP70‑PCs healed faster than negative control cells. HSP70/HSP70‑PCs‑treated cells also exhibited better migration ability and higher invasion ability than control cells. HSP70/HSP70‑PCs treatment did not alter the messenger RNA (mRNA) or protein levels of matrix metalloproteinase‑9; the opposite was true for Ras homolog family member A (RhoA) mRNA and protein levels. RNA interference of RhoA attenuated the migration of HSP70/HSP70‑PCs‑treated cells. The present findings indicate that regulation of HCC cell migration by HSP70/HSP70‑PCs occurs via regulation of RhoA expression.

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Journal Cover

March 2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

2015 Impact Factor: 1.482
Ranked #97/211 Oncology
(total number of cites)

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APA
Yi, Z., Li, Y., Liu, D., Liu, J., & Li, H. (2017). Extracellular HSP70/HSP70‑PCs regulate hepatocarcinoma cell migration and invasion via RhoA. Oncology Letters, 13, 1095-1100. http://dx.doi.org/10.3892/ol.2016.5551
MLA
Yi, Z., Li, Y., Liu, D., Liu, J., Li, H."Extracellular HSP70/HSP70‑PCs regulate hepatocarcinoma cell migration and invasion via RhoA". Oncology Letters 13.3 (2017): 1095-1100.
Chicago
Yi, Z., Li, Y., Liu, D., Liu, J., Li, H."Extracellular HSP70/HSP70‑PCs regulate hepatocarcinoma cell migration and invasion via RhoA". Oncology Letters 13, no. 3 (2017): 1095-1100. http://dx.doi.org/10.3892/ol.2016.5551