Extracellular HSP70/HSP70‑PCs regulate hepatocarcinoma cell migration and invasion via RhoA
- Zhe Yi
- Yan Li
- Dan Liu
- Jingang Liu
- Hangyu Li
Published online on: Friday, December 30, 2016
The effects of heat shock protein 70 (HSP70)/HSP70‑peptide complexes (HSP70‑PCs) on the invasion and metastasis ability of hepatocellular carcinoma (HCC) Huh‑7 cells were investigated. Wound healing assay revealed that cells treated with HSP70/HSP70‑PCs healed faster than negative control cells. HSP70/HSP70‑PCs‑treated cells also exhibited better migration ability and higher invasion ability than control cells. HSP70/HSP70‑PCs treatment did not alter the messenger RNA (mRNA) or protein levels of matrix metalloproteinase‑9; the opposite was true for Ras homolog family member A (RhoA) mRNA and protein levels. RNA interference of RhoA attenuated the migration of HSP70/HSP70‑PCs‑treated cells. The present findings indicate that regulation of HCC cell migration by HSP70/HSP70‑PCs occurs via regulation of RhoA expression.