Extracellular HSP70/HSP70‑PCs regulate hepatocarcinoma cell migration and invasion via RhoA

Yi,Zhe; Li,Yan; Liu,Dan; Liu,Jingang; Li,Hangyu

doi:10.3892/ol.2016.5551

Extracellular HSP70/HSP70‑PCs regulate hepatocarcinoma cell migration and invasion via RhoA

Authors:
- Zhe Yi
- Yan Li
- Dan Liu
- Jingang Liu
- Hangyu Li
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Affiliations: School of Stomatology, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China
Published online on: December 30, 2016 https://doi.org/10.3892/ol.2016.5551
Pages: 1095-1100
Copyright: © Yi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The effects of heat shock protein 70 (HSP70)/HSP70‑peptide complexes (HSP70‑PCs) on the invasion and metastasis ability of hepatocellular carcinoma (HCC) Huh‑7 cells were investigated. Wound healing assay revealed that cells treated with HSP70/HSP70‑PCs healed faster than negative control cells. HSP70/HSP70‑PCs‑treated cells also exhibited better migration ability and higher invasion ability than control cells. HSP70/HSP70‑PCs treatment did not alter the messenger RNA (mRNA) or protein levels of matrix metalloproteinase‑9; the opposite was true for Ras homolog family member A (RhoA) mRNA and protein levels. RNA interference of RhoA attenuated the migration of HSP70/HSP70‑PCs‑treated cells. The present findings indicate that regulation of HCC cell migration by HSP70/HSP70‑PCs occurs via regulation of RhoA expression.

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