Decreased soluble RAGE in neutrophilic asthma is correlated with disease severity and RAGE G82S variants
- Authors:
- Published online on: December 18, 2017 https://doi.org/10.3892/mmr.2017.8302
- Pages: 4131-4137
Abstract
Introduction
The advanced glycosylation end product-specific receptor (RAGE) is a single-transmembrane, multi-ligand receptor that belongs to the immunoglobulin superfamily. It was initially cloned in 1992 and termed receptor for advanced glycosylation end products (AGEs) of proteins (1).
The RAGE gene generates extensive splice variants in humans and other mammals (2,3). In addition to the full-length RAGE, there are two major splice variants: Variant 1 (C-truncated RAGE) and variant 2 (N-truncated RAGE). N-truncation deletes the V-type domain, the ligand binding domain, from the full-length RAGE. C-truncation removes the transmembrane and C-terminal domains of the full-length RAGE and contains only the extracellular domain of RAGE (2), rendering RAGE free (secreted) and soluble. One variant of soluble RAGE (sRAGE), endogenous secretory RAGE (esRAGE), also termed RAGE variant 1, is generated through alternative RNA splicing (4,5). In humans, full-length RAGE is the most prevalent form of RAGE, followed by esRAGE, the most predominant soluble form of the sRAGE isoforms (2,5). In addition to alternative RNA splicing, sRAGE may be generated via proteolytic cleavage of the full-length RAGE (6,7). This type of RAGE is termed cleaved RAGE (cRAGE). However, esRAGE and cRAGE are able to bind the same RAGE ligands, and are therefore functionally equivalent (5).
RAGE binds a variety of ligands, including AGEs, high mobility group protein B1 (HMGB1), serum amyloid A (SAA), and S100 family proteins (8–11). RAGE is highly expressed in normal lungs, particularly in alveolar epithelial type I cells, compared with other tissues (12,13). A recent study demonstrated that the absence of RAGE abolished the majority of the typical pathological indicators of asthma, including airway hypersensitivity, eosinophilic inflammation and airway remodeling, in a house dust mite-RAGE knockout mouse model of asthma/allergic airway disease, suggesting that RAGE may be a principal mediator of asthma pathogenesis (14). In addition, increased expression of RAGE ligands in asthma has been reported, including HMGB1, SAA and S100 family proteins (8–11). Conceivably, suppression of RAGE activation and/or expression may be a promising approach to decrease the airway inflammation commonly observed in asthma.
sRAGE may act as a decoy receptor to compete with RAGE ligands for binding with the full-length RAGE, thereby suppressing the activation of RAGE at the cell surface (15). Accumulating evidence has demonstrated that sRAGE may be associated with various chronic diseases, including metabolic, cardiovascular, neurodegenerative and inflammatory disorders (16–19). Previous studies have demonstrated that sRAGE was decreased in patients with chronic obstructive pulmonary disease (COPD) and was correlated with the severity of emphysema and neutrophilic inflammatory conditions (20–25).
RAGE genetic variability additionally results from single-nucleotide polymorphisms (SNPs). A total of >30 SNPs have been identified within the RAGE gene and are associated with diabetes mellitus (26). Among these SNPs, RAGE genetic variation rs2070600 (Gly82Ser, G82S), which causes a glycine to serine substitution at amino acid number 82 of the ligand-binding V-domain (Gly82Ser) in the extracellular domain, is of particular interest, as it has been associated with circulating RAGE levels (27,28) and COPD (21). In addition, genome-wide association studies have revealed an association between the Gly82Ser polymorphism and pulmonary function (29,30).
However, to date, there have been few studies, which have examined the association between sRAGE and asthma. These studies had small sample sizes and their results were contradictory (11,20,31). For example, Sukkar et al (20) reported that sRAGE was deficient in neutrophilic asthma. Other studies, however, reported elevated levels of sRAGE in asthmatics (11,31). The potential reason for the discrepancy may be attributable to the fact that different asthma inflammatory phenotypes, which are classified as neutrophilic and non-neutrophilic based on induced sputum inflammatory cells (20), are associated with RAGE genetic variants.
Therefore, the primary purpose of the present study was to determine plasma sRAGE levels in asthmatics with different inflammatory phenotypes, correlate plasma sRAGE levels with related clinical characteristics, and assess the association between plasma sRAGE and the RAGE G82S genetic variant.
Materials and methods
Study population
A total of 96 patients were recruited from the outpatient clinic of Nanfang Hospital, Southern Medical University (Guangzhou, China), between September 2011 and September 2012, and 97 patients from The People's Hospital of Zhongshan City (Zhongshan, China) between September 2014 and December 2015. The characteristics of the subjects are displayed in Table I. All patients were >18 years old and newly-diagnosed with asthma via positive bronchodilator reversibility test or airway hyper-responsiveness to methacholine. A positive bronchodilator reversibility test is defined as an increase in forced expiratory volume in 1 sec (FEV1) of >12% and >200 ml from baseline, 10–15 min subsequent to the inhalation of 200–400 μg albuterol or equivalent. Airway hyper-responsiveness to methacholine is defined as a decrease in FEV1 from baseline of ≥20% with standard doses of methacholine (32). As a control group, 118 healthy individuals without a history of chronic respiratory disease, allergies, cardiovascular disease, diabetes and neurological diseases, and without evidence of airflow obstruction measured by spirometry, were recruited between September 2014 and December 2015. The present study was approved by the Medical Ethics Committees of Nanfang Hospital and The People's Hospital of Zhongshan City. All subjects provided written informed consent for participation in the study.
Study design
All participants completed a questionnaire to collect data about age, sex, weight, height and smoking history. Nonsmokers were defined as those who had a <2-pack-year (pack-year, cigarette packs per day multiplied by the number of years smoking) smoking history during their lifetime, and had not smoked for at least 1 year prior to participation. Smokers were defined as having a smoking history of 2 pack-years or more. All participants received pulmonary function testing, sputum induction and collection of a 5-ml EDTA-preserved blood specimen following recruitment. All asthmatics were divided into a neutrophilic asthma group (neutrophils ≥65%) and non-neutrophilic asthma group (neutrophils <65%) based on induced sputum inflammatory cells. This 65% cut-off value was selected according to a previous report (20).
Sample preparation and measurement of sRAGE
Blood collection and sputum induction and their processing were performed as per a previous report (8). Sputum smears in which the ratio of squamous epithelial cells/nuclear cells was <20% were considered to be adequate specimens. Following processing of the blood samples, plasma was stored at −80°C until use. Plasma sRAGE levels were detected by ELISA analysis using Quantikine sRAGE ELISA, (cat. no. DRG00; R&D Systems, Inc., Minneapolis, MN, USA), according to the manufacturer's protocol. This ELISA kit measures the total pool of sRAGE, including esRAGE and cRAGE (2).
DNA preparation and genotype analyses of RAGE G82S
Genomic DNA was prepared from peripheral blood samples using a pure gene DNA purification kit (Tiangen Biotech Co., Ltd., Beijing, China), according to the manufacturer's protocol. The RAGE SNP (rs2070600; G82S) was determined using the Sanger sequencing method by BGI (Shenzhen, China).
Statistical analysis
SPSS 16.0 software (SPSS, Inc., Chicago, IL, USA) was used for statistical analysis. Continuous variables were evaluated for a normal distribution using the Kolmogorov-Smirnov test. Parametric data are presented as mean ± standard deviation. Categorical variables are presented as either medians (interquartile ranges) or numbers (percentages), as appropriate. Comparisons among groups were performed using one-way analysis of variance. For the post hoc multiple comparisons, least significant difference test was used when equal variances were assumed and Tamhane's T2 test was used when equal variances were not assumed. Categorical variables were examined using the χ2 test. Correlations were assessed using Spearman or Pearson rank correlation coefficients. The Hardy-Weinberg equilibrium of the G82S polymorphism was examined using the Executive SNP Analyzer 1.0 (BGI, Shenzhen, China). All statistical analyses were two-sided, and P<0.05 was considered to indicate a statistically significant difference.
Results
Subject characteristics
A total of 194 newly-diagnosed asthmatics and 118 healthy controls were included in the analysis. Of the asthma patients, 85 were neutrophilic and 109 were non-neutrophilic. The characteristics of the subjects are displayed in Table I. There was no significant difference in sex, body mass index (BMI) and smoking history between asthmatic and control subjects. The mean age of the non-neutrophilic asthma group was lower compared with that of the neutrophilic and control groups. Asthmatics had a decreased FEV1% predicted (FEV1% Pre) compared with healthy controls, although there was no difference between the two asthma groups.
Plasma sRAGE levels
There was a significant difference in plasma sRAGE levels between asthmatic patients and normal controls (F=15.925; P<0.001). The mean plasma sRAGE level was significantly decreased in neutrophilic asthmatics (443.67±208.9 pg/ml) and increased in non-neutrophilic asthmatics (677.63±300.75 pg/ml), compared with the control group (550.02±300.83 pg/ml; Fig. 1).
Correlation of plasma sRAGE with clinical characteristics of asthmatic patients
The plasma sRAGE level of neutrophilic asthmatics was positively correlated with FEV1% Pre (rp=0.258; P=0.023; Fig. 2A), while no significant correlation was observed in non-neutrophilic asthmatics (P=0.406). The plasma sRAGE level of non-neutrophilic asthmatics was inversely correlated with the percentage of sputum neutrophils (rp=−0.278; P=0.009; Fig. 2B), although no significant correlation was observed in neutrophilic asthmatics (P=0.183). There was no significant correlation between plasma sRAGE level and sex, BMI, age, smoking history or and FVC% Pre, or the percentage of sputum eosinophils (P>0.05), in the two asthma groups.
Frequencies of RAGE G82S polymorphism
The RAGE G82S genotype distribution among neutrophilic asthmatics (G/G, 50.6%; A/G, 45.9%; A/A, 3.5%), non-neutrophilic asthmatics (G/G, 69.7%; A/G, 24.8%; A/A, 5.5%), and controls (G/G, 69.5%; A/G, 26.3%; A/A, 4.2%) did not deviate significantly from Hardy-Weinberg equilibrium (χ2=2.71, P=0.100; χ2=2.682, P=0.102; and χ2=0.851, P=0.356, respectively) (Fig. 3). The frequency of the G82S genotype was significantly different between neutrophilic and non-neutrophilic asthmatics (χ2=9.510, P=0.009), and between neutrophilic asthmatics and healthy controls (χ2=8.441, P=0.015), while no significant difference between non-neutrophilic asthmatics and healthy controls was noted (χ2=0.238, P=0.888).
Association of RAGE G82S genotypes with plasma sRAGE and FEV1% Pre
The present study analyzed the association between RAGE G82S genotypes and plasma sRAGE distribution (Fig. 4A), and demonstrated a significant association between G82S genotypes and plasma sRAGE levels in neutrophilic asthmatics. Following adjustment for age, subjects with the G/G genotype exhibited a significantly increased sRAGE level (498.64±235.37 pg/ml) compared with those with the A/G genotype (389.83±150.37 pg/ml) and A/A genotype (264.59±161.74 pg/ml) (P=0.022). Only 3 neutrophil subjects had an A/A genotype, and they had very low plasma sRAGE levels. Neutrophilic asthmatics with the RAGE A/G or A/A genotype displayed a trend toward lower FEV1% Pre compared with those with the G/G genotype (60.14±22.36% vs. 64.51±27.37%; Fig. 4B). However, significant differences in sRAGE between the different genotypes, and associations with FEV1% Pre, were not observed in non-neutrophilic asthmatics and healthy controls.
Discussion
The present study demonstrated that plasma sRAGE levels were altered in different asthma inflammatory phenotypes. At initial diagnosis, plasma sRAGE levels were decreased in patients with neutrophilic asthma and increased in patients with non-neutrophilic asthma compared with healthy subjects. In addition, the plasma sRAGE level was positively correlated with FEV1% Pre in neutrophilic asthmatics. In terms of G82S genotype, the frequencies of G82S genotypes were different between neutrophilic asthmatics and healthy controls. G82S genotype exhibited a trend that correlated with the severity of pulmonary dysfunction. To the best of our knowledge, the present study was the first to report a likely association between RAGE G82S gene variants and plasma sRAGE levels in patients with asthma.
Few studies have examined sRAGE levels and asthma inflammatory phenotypes. In accordance with the results of the present study, Watanabe et al (11) reported elevated levels of sRAGE in the sputum of asthmatic patients. In that study, the subjects exhibited low levels of sputum neutrophils (median, 24%; upper limit, 47%), and high eosinophil counts (median, 9.4%; lower limit, 4.5%). However, Sukkar et al (20) reported that sRAGE was deficient in neutrophilic asthma, which is consistent with the results of the present study. Notably, the patients in the study of Sukkar et al (20) were clinically stable older asthmatics, and 68% of them used inhaled corticosteroids; in the present study, all the patients were newly-diagnosed asthmatics, suggesting that plasma sRAGE levels, although altered in different asthma inflammatory phenotypes, may remain relatively stable regardless of medical therapy. Similar to the findings adults (7,11,16,20), Eli-Seify et al (33) reported that children with >2% eosinophils and ≥40% neutrophils exhibited lower sRAGE levels compared with children with >2% eosinophils and <40% neutrophils; this suggests that the sRAGE level may remain relatively stable in different asthma inflammatory phenotypes regardless of age.
Previous studies have demonstrated that decreased plasma levels in sRAGE were associated with increased progression of airflow limitations in patients with COPD patients and asthmatic children (24,33). However, Watanabe et al (11) and Zhou et al (31) observed that sRAGE levels were not associated with asthma severity in adult patients with asthma, possibly as their patients were all non-neutrophilic asthmatics. In the present study, no significant association between plasma sRAGE levels and asthma severity was observed in non-neutrophilic asthmatics. However, it was demonstrated that decreased plasma sRAGE levels were significantly associated with FEV1% Pre in neutrophilic asthmatics, suggesting that plasma sRAGE may be a potential systemic biomarker for neutrophilic asthma severity, although not for patients with non-neutrophilic asthma.
In the present study, the sRAGE levels measured comprised all types of sRAGE, including esRAGE and cRAGE. esRAGE has been identified to be the primary soluble variant of RAGE, as demonstrated by Sukkar et al (20) in neutrophilic and non-neutrophilic asthmatics. esRAGE is generated as a splice variant of the RAGE gene. The association of the G82S variant with blood sRAGE levels has been previously reported in a number of diseases, including diabetes mellitus and COPD (21,27,34). In the present study, it was demonstrated that the G82S variant was significantly correlated with plasma sRAGE levels, patients with the A allele had lower plasma sRAGE levels, and G82S variant was correlated with the severity of pulmonary dysfunction in neutrophilic asthmatics. By contrast, these findings were not observed in non-neutrophilic asthmatics and healthy controls. Furthermore, in a genome wide association study performed in a Japanese population, the RAGE G82S variant was reported to not be significantly associated with asthma susceptibility, although another single nucleotide polymorphism (rs404860) located in close proximity was associated with asthma susceptibility (35). These results raised the possibility that sRAGE levels may be determined by genetic variation in the RAGE locus, and the RAGE locus may be differentially regulated between neutrophilic and non-neutrophilic asthma patients.
sRAGE may be consumed by competitively binding to the ligands of RAGE, including HMGB1 and SAA; therefore, the levels of RAGE ligands may influence plasma sRAGE levels. Previous studies have reported that plasma and sputum HMGB1 and SAA were increased in patients with asthma and COPD, and correlated with higher degrees of airway neutrophilia and systemic inflammation (20,25,36–38). Neutrophilic asthmatics exhibited more severe airway neutrophil inflammation compared with non-neutrophilic asthmatics, and it was hypothesized that a higher percentage of sputum neutrophils and increased RAGE ligands may be additional reasons for the decreased plasma sRAGE levels in neutrophilic asthmatics. However, no significant correlation was observed between plasma sRAGE levels and the percentage of sputum neutrophils in neutrophilic asthmatics, and it was previously reported that there were no significant differences in plasma HMGB1 levels between different asthma inflammatory phenotypes (8). These results further suggested that the G82S variant may be the primary reason for decreased plasma sRAGE levels.
It is unknown why the plasma sRAGE level was increased in non-neutrophilic asthmatics. In the present study, no significant association was observed between plasma sRAGE levels and G82S variants. It was demonstrated that the plasma sRAGE level was inversely correlated with the percentage of sputum neutrophils in non-neutrophilic asthmatics; however, a higher percentage of sputum neutrophils has been previously reported to be associated with lower plasma sRAGE levels (20,39), thus it may be hypothesized that there may exist other factors which influence the sRAGE level in non-neutrophilic asthmatics. In addition to esRAGE, sRAGE has another isoform, cRAGE, which is produced via proteolytic cleavage of membrane-bound RAGE by metalloproteinases (6,7). Increased expression levels of RAGE and metalloproteinases in airway and inflammatory cells may induce an increase in plasma sRAGE in asthmatic patients. However, no research into this has yet been reported.
There are a number of limitations to the present study. The sample size was relatively small. The levels of RAGE ligands were not examined, which may influence the plasma sRAGE level. In addition, the reasons for the increased plasma sRAGE level in non-neutrophilic asthmatics were not determined.
In conclusion, plasma sRAGE is decreased in neutrophilic asthmatics and is correlated with the severity of neutrophilic asthma and G82S genotype variant. The importance of and the mechanism underlying the increased plasma sRAGE level in non-neutrophilic asthmatics requires further study.
Acknowledgements
The present study was supported by grants from the National Natural Science Foundation of China (grant nos. 81470228 and 81300029), the National Program on Key Basic Research Project (973 Program; grant no. 2012CB518203), the Natural Science Foundation of Guangdong Province (grant no. 2015A030313236), the Scientific and Technological Projects of Guangdong Province (grant no. 2016A020215117), the Medicine Foundation of Guangdong Province (grant no. A2015007), and the Science and Technology Program of Zhongshan City (grant no. 2015B1103).
Glossary
Abbreviations
Abbreviations:
RAGE |
advanced glycosylation end product-specific receptor |
sRAGE |
soluble advanced glycosylation end product-specific receptor |
esRAGE |
endogenous secretory advanced glycosylation end product-specific receptor |
cRAGE |
cleaved advanced glycosylation end product-specific receptor |
AGE |
advanced glycosylation end product |
HMGB1 |
high mobility group protein B1 |
FEV1 |
forced expiratory volume in 1 sec |
COPD |
chronic obstructive pulmonary disease |
BMI |
body mass index |
SAA |
serum amyloid A |
SNP |
single nucleotide polymorphism |
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