In vitro effect of molluscan hemocyanins on CAL‑29 and T‑24 bladder cancer cell lines

Boyanova,Olga; Dolashka,Pavlina; Toncheva,Draga; Rammensee,Hans‑Georg; Stevanović,Stefan

doi:10.3892/br.2012.46

In vitro effect of molluscan hemocyanins on CAL‑29 and T‑24 bladder cancer cell lines

Authors:
- Olga Boyanova
- Pavlina Dolashka
- Draga Toncheva
- Hans‑Georg Rammensee
- Stefan Stevanović
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Affiliations: Department of Medical Genetics, Medical University of Sofia, Sofia 1431, Bulgaria, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria, Institute for Cell Biology, Department of Immunology, University of Tübingen, D‑72076 Tübingen, Germany
Published online on: December 10, 2012 https://doi.org/10.3892/br.2012.46
Pages: 235-238

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Abstract

The aim of this study was to investigate the antitumor effects of molluscan hemocyanins (Hcs) isolated from the marine snail Rapana venosa (RvH) and the garden snail Helix lucorum (HlH) on human bladder cancer cell lines. The antitumor effect of the native molecules of the above‑mentioned Hcs and their subunits were examined in comparison to keyhole limpet hemocyanin (KLH), which is the most thoroughly studied Hc. The experiments were conducted using 2 human bladder cancer cell lines: CAL‑29 and T‑24. Doxorubicin hydrochloride (DOX) and mitomycin‑C (MIT‑C), which are routinely used in clinical practice to treat bladder cancer, were used for comparison. The viability of the 2 bladder cancer cell lines, used at a concentration of 20,000 cells/well, was measured by WST‑1 assay at 24, 48 and 72 h after treatment with the above‑mentioned Hcs and their isoforms at a concentration ranging from 0.8 to 500 µg/ml. A direct growth inhibitory effect on the tumor cells was observed mainly after treatment with the native molecule of HlH and the structural subunit, RvH1, at a concentration of 500 µg/ml. The native molecule of RvH exhibited an efficacy similar to that of KLH. However, the observed growth inhibitory effect of HlH was superior to that observed for KLH and RvH, when used at the same concentration. These findings demonstrate the antitumor effect of other Hcs, apart from KLH. Our data suggest that the native molecule of HlH and the subunit, RvH1, are alternative candidates for the treatment of human superficial bladder cancer.

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