High glucose‑induced resistance to 5-fluorouracil in pancreatic cancer cells alleviated by 2-deoxy-D-glucose

  • Authors:
    • Yao Cheng
    • Dongmei Diao
    • Hao Zhang
    • Qi Guo
    • Xuandi Wu
    • Yongchun Song
    • Chengxue Dang
  • View Affiliations

  • Published online on: December 9, 2013     https://doi.org/10.3892/br.2013.211
  • Pages: 188-192
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Abstract

Abnormal glucose metabolism from hyperglycemia or diabetes aggravates the progression of pancreatic cancer. It is unknown whether high glucose has an impact on the antitumor effect of 5-fluorouracil (5-Fu) and whether targeting aberrant glucose metabolism using 2-deoxy-D-glucose (2-DG) may reverse this effect in high‑glucose microenvironments. The cell viability of AsPC-1 and Panc-1 was analyzed by MTT assay following 5-Fu treatment at different glucose concentrations. Altered sensitivity to 5-Fu by 2-DG was also analyzed. LY294002 was used to inhibit PI3K-Akt signaling to determine the mechanism involved. In response to glucose, 5-Fu‑induced cell growth inhibition was attenuated in a dose‑dependent manner, accompanied with activated p-Akt, while 2-DG enhanced 5-Fu‑induced cell growth inhibition. Moreover, blocking the PI3K/Akt pathway by LY294002 effectively eliminated 2-DG‑induced apoptosis. In conclusion, high glucose weakens the antitumor effect of 5-Fu via PI3K/Akt signaling. Using 2-DG in combination with 5-Fu significantly increased their therapeutic effectiveness in high‑glucose microenvironments.
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March-April 2014
Volume 2 Issue 2

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Spandidos Publications style
Cheng Y, Diao D, Zhang H, Guo Q, Wu X, Song Y and Dang C: High glucose‑induced resistance to 5-fluorouracil in pancreatic cancer cells alleviated by 2-deoxy-D-glucose. Biomed Rep 2: 188-192, 2014
APA
Cheng, Y., Diao, D., Zhang, H., Guo, Q., Wu, X., Song, Y., & Dang, C. (2014). High glucose‑induced resistance to 5-fluorouracil in pancreatic cancer cells alleviated by 2-deoxy-D-glucose. Biomedical Reports, 2, 188-192. https://doi.org/10.3892/br.2013.211
MLA
Cheng, Y., Diao, D., Zhang, H., Guo, Q., Wu, X., Song, Y., Dang, C."High glucose‑induced resistance to 5-fluorouracil in pancreatic cancer cells alleviated by 2-deoxy-D-glucose". Biomedical Reports 2.2 (2014): 188-192.
Chicago
Cheng, Y., Diao, D., Zhang, H., Guo, Q., Wu, X., Song, Y., Dang, C."High glucose‑induced resistance to 5-fluorouracil in pancreatic cancer cells alleviated by 2-deoxy-D-glucose". Biomedical Reports 2, no. 2 (2014): 188-192. https://doi.org/10.3892/br.2013.211