A serum metabolomic investigation on lipoprotein lipase‑deficient mice with hyperlipidemic pancreatitis using gas chromatography/mass spectrometry

Tang,Maochun; Hu,Gouyong; Zhao,Yan; Su,Mingming; Wang,Yuhui; Jia,Wei; Qiu,Yunping; Liu,George; Wang,Xingpeng

doi:10.3892/br.2013.78

May-June 2013 Volume 1 Issue 3

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May-June 2013 Volume 1 Issue 3

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Article

A serum metabolomic investigation on lipoprotein lipase‑deficient mice with hyperlipidemic pancreatitis using gas chromatography/mass spectrometry

Authors:
- Maochun Tang
- Gouyong Hu
- Yan Zhao
- Mingming Su
- Yuhui Wang
- Wei Jia
- Yunping Qiu
- George Liu
- Xingpeng Wang
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Affiliations: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, P.R. China, Institute of Cardiovascular Sciences and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Peking University, Beijing 100083, P.R. China, Shanghai Institute for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, P.R. China
Pages: 469-473
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Published online on: March 12, 2013

https://doi.org/10.3892/br.2013.78
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Abstract

Hypertriglyceridemia (HTG) is often associated with acute pancreatitis. The relationship between these diseases and the role that hypertriglyceridemia plays in acute pancreatitis pathogenesis remains to be elucidated. In the present study, in order to investigate the mechanisms of hyperlipidemic acute pancreatitis (HLP), we established an animal model using lipoprotein lipase (LPL)‑deficient heterozygous mice injected with caerulein. Susceptibility to pancreatitis in LPL‑deficient heterozygous mice was compared with that of wild‑type mice after intraperitoneal (i.p.) injections of caerulein by determining amylase release and pancreatic pathological scores. Furthermore, serum metabolome was detected through chemical derivatization followed by gas chromatography/mass spectrometry (GC/MS). GC/MS data were analyzed by orthogonal‑projection to latent structures‑discriminant analysis (OPLS‑DA). Caerulein induced increased levels of serum amylase and more severe pancreatic inflammation in LPL‑deficient mice compared to wild‑type mice. HLP was discriminated more accurately from healthy controls by the metabolites, including valine, leucine and citrate. The metabolites included valine, leucine, citrate, malic acid, proline, tetradecanoic acid (14:0), glutamine and oleic acid (18:1). Changes in energy, fat and amino acid metabolism were also evident. In conclusion, LPL‑deficient heterozygous mice with hypertriglyceridemia (HTG) exhibited enhanced susceptibility to acute pancreatitis. GC/MS data revealed differences between healthy and HLP mice. Therefore, this technique is novel and a useful tool for the study of the HLP pathogenetic mechanism.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A serum metabolomic investigation on lipoprotein lipase‑deficient mice with hyperlipidemic pancreatitis using gas chromatography/mass spectrometry

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