Synergistic antioxidant activity of resveratrol with genistein in high-glucose treated Madin-Darby canine kidney epithelial cells

Chu,Chishih; Lu,Fung‑Jou; Yeh,Rang‑Hui; Li,Zih‑Ling; Chen,Ching‑Hsein

doi:10.3892/br.2016.573

Synergistic antioxidant activity of resveratrol with genistein in high-glucose treated Madin-Darby canine kidney epithelial cells

Authors:
- Chishih Chu
- Fung‑Jou Lu
- Rang‑Hui Yeh
- Zih‑Ling Li
- Ching‑Hsein Chen
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Affiliations: Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi 60004, Taiwan, R.O.C., Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan, R.O.C., Department of Physical Medicine and Rehabilitation, Chia‑Yi Chang Gung Memorial Hospital, Chiayi 61363, Taiwan, R.O.C.
Published online on: January 19, 2016 https://doi.org/10.3892/br.2016.573
Pages: 349-354

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Abstract

Resveratrol (Re), a stilbenoid, is associated with a potential benefit in controlling certain biomarkers in type II diabetes. Genistein (Ge), a phytoestrogen, may act as an antioxidant and thus may diminish damaging effects of free radicals in tissues. In the present study, a potential synergistic antioxidant effect of an Re/Ge combination on high‑glucose (HG) incubation in Madin‑Darby canine kidney (MDCK) epithelial cells was evaluated. Compared with the treatment of Re or Ge alone, the Re/Ge combination synergistically decreased intracellular reactive oxygen species (ROS) and hydroxyl radicals in MDCK cells. This synergistic antioxidant effect correlated with the inhibition of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase expression and an increase in γ‑glutamylcysteine synthetase expression. In addition, mitochondrial complex I, NADPH oxidase, xanthine oxidase and lipoxygenase contributed towards ROS overproduction when the MDCK cells were incubated with HG. In conclusion, the Re/Ge combination synergistically enhanced the antioxidant effect in HG‑incubated kidney cells, possibly through an enhanced antioxidant regulation mechanism. The Re/Ge combination may be a potential benefit against oxidative stress in diabetes mellitus.

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