Rosehip inhibits xanthine oxidase activity and reduces serum urate levels in a mouse model of hyperuricemia

  • Authors:
    • Hidetomo Kikuchi
    • Satomi Kogure
    • Rie Arai
    • Kouki Saino
    • Atsuko Ohkubo
    • Tadashi Tsuda
    • Katsuyoshi Sunaga
  • View Affiliations

  • Published online on: April 10, 2017     https://doi.org/10.3892/br.2017.888
  • Pages: 539-544
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Abstract

Rosehip, the fruit of Rosa canina L., has traditionally been used to treat urate metabolism disorders; however, its effects on such disorders have not been characterized in detail. Therefore, the present study investigated the effects of hot water, ethanol and ethyl acetate extracts of rosehip on xanthine oxidase (XO) activity in vitro. In addition, the serum urate lowering effects of the rosehip hot water extract in a mouse model of hyperuricemia (male ddY mice, which were intraperitoneally injected with potassium oxonate) were investigated. Furthermore, the influence of rosehip hot water extract on CYP3A4 activity, which is the most important drug‑metabolizing enzyme from a herb‑drug interaction perspective, was investigated. Rosehip extracts of hot water, ethanol and ethyl acetate inhibited XO activity [half maximal inhibitory concentration (IC50) values: 259.6±50.6, 242.5±46.2 and 1,462.8±544.2 µg/ml, respectively]. Furthermore, the administration of 1X rosehip hot water extract significantly reduced the levels of serum urate at 8 h, which was similar when compared with the administration of 1 mg/kg allopurinol. Rosehip hot water extract only marginally affected CYP3A4 activity (IC50 value, >1 mg/ml). These findings indicate that rosehip hot water extract may present as a functional food for individuals with a high urate level, and as a therapeutic reagent for hyperuricemic patients.
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May-2017
Volume 6 Issue 5

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Kikuchi H, Kogure S, Arai R, Saino K, Ohkubo A, Tsuda T and Sunaga K: Rosehip inhibits xanthine oxidase activity and reduces serum urate levels in a mouse model of hyperuricemia. Biomed Rep 6: 539-544, 2017.
APA
Kikuchi, H., Kogure, S., Arai, R., Saino, K., Ohkubo, A., Tsuda, T., & Sunaga, K. (2017). Rosehip inhibits xanthine oxidase activity and reduces serum urate levels in a mouse model of hyperuricemia. Biomedical Reports, 6, 539-544. https://doi.org/10.3892/br.2017.888
MLA
Kikuchi, H., Kogure, S., Arai, R., Saino, K., Ohkubo, A., Tsuda, T., Sunaga, K."Rosehip inhibits xanthine oxidase activity and reduces serum urate levels in a mouse model of hyperuricemia". Biomedical Reports 6.5 (2017): 539-544.
Chicago
Kikuchi, H., Kogure, S., Arai, R., Saino, K., Ohkubo, A., Tsuda, T., Sunaga, K."Rosehip inhibits xanthine oxidase activity and reduces serum urate levels in a mouse model of hyperuricemia". Biomedical Reports 6, no. 5 (2017): 539-544. https://doi.org/10.3892/br.2017.888