Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells

  • Authors:
    • Chung-Lung Cho
    • Ya-Zhe Lee
    • Chao-Neng Tseng
    • Joshua Cho
    • Yuan-Bin Cheng
    • Kuo‑Wei Wang
    • Han-Jung Chen
    • Shean-Jaw Chiou
    • Chia-Hua Chou
    • Yi-Ren Hong
  • View Affiliations

  • Published online on: September 11, 2017     https://doi.org/10.3892/br.2017.979
0

Abstract

Pluchea indica (L.) Less. is a perennial plant known for its versatile uses in traditional medicine. Previous findings have shown that the extracts of Pluchea indica possess significant anti-inflammatory, anti-ulcer and anti-tuberculosis activity. The aim of this study was to demonstrate the anticancer activity of the hexane fraction of P. indica root extract (H-PIRE) in human glioblastoma cells using flow cytometric and western blot analysis. The results shoewd that, H-PIRE suppressed the growth of glioblastoma cells in a dose-dependent manner. H-PIRE treatment markedly decreased the population of cells in S and G2/M phases. The significant upregulation of acidic vesicular organelle (AVO) was detected during H-PIRE treatment. The expression levels of microtubule-associated light chain 3-II (LC3-II) protein, phosphorylated JNK and phosphorylated p38 were significantly increased, confirming the occurrence of autophagy during the process. Finally, the combination treatment of H-PIRE and LY294002, a pan PI3K inhibitor, further decreased cell viability, suggesting an additive anticancer effect. Taken together, our results suggest that H-PIRE suppresses the proliferation of glioblastoma cells by inducing cell cycle arrest and autophagy.

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APA
Cho, C., Lee, Y., Tseng, C., Cho, J., Cheng, Y., Wang, K. ... Hong, Y. (1899). Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells. Biomedical Reports, 0, 0-0. https://doi.org/10.3892/br.2017.979
MLA
Cho, C., Lee, Y., Tseng, C., Cho, J., Cheng, Y., Wang, K., Chen, H., Chiou, S., Chou, C., Hong, Y."Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells". Biomedical Reports 0.0 (1899): 0-0.
Chicago
Cho, C., Lee, Y., Tseng, C., Cho, J., Cheng, Y., Wang, K., Chen, H., Chiou, S., Chou, C., Hong, Y."Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells". Biomedical Reports 0, no. 0 (1899): 0-0. https://doi.org/10.3892/br.2017.979