The ‑2549 insertion/deletion polymorphism in the promoter region of VEGF is associated with the risk of recurrent spontaneous abortion

Hashemi,Mohammad; Danesh,Hiva; Bizhani,Fatemeh; Mokhtari,Mojgan; Bahari,Gholamreza; Tabasi,Farhad; Taheri,Mohsen

doi:10.3892/br.2018.1050

The ‑2549 insertion/deletion polymorphism in the promoter region of VEGF is associated with the risk of recurrent spontaneous abortion

Authors:
- Mohammad Hashemi
- Hiva Danesh
- Fatemeh Bizhani
- Mojgan Mokhtari
- Gholamreza Bahari
- Farhad Tabasi
- Mohsen Taheri
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Affiliations: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 98167‑43181, Iran, Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167‑43181, Iran, Department of Obstetrics and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167‑43181, Iran, Student Research Committee, Zahedan University of Medical Sciences, Zahedan 98167‑43181, Iran, Department of Genetics, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167‑43181, Iran
Published online on: January 30, 2018 https://doi.org/10.3892/br.2018.1050
Pages: 297-300

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Abstract

Recurrent spontaneous abortion (RSA) is a common health problem affecting women of reproductive age. Altered expression of vascular endothelial growth factor (VEGF) has been associated with spontaneous abortion. The present case‑control study aimed to evaluate the impact of the 18‑bp insertion/deletion (ins/del) polymorphism (rs35569394) in the promoter region of the VEGF gene on idiopathic RSA. Genomic DNA from 93 patients with RSA and 93 healthy fertile women of southeastern Iran was isolated using the salting‑out method. Genotyping of the rs35569394 variant was performed by a polymerase chain reaction (PCR) method. The findings indicated that the VEGF 18‑bp ins/del variant significantly increased the risk of RSA under codominant (ins/ins vs. del/del; OR=2.85, 95% CI=1.31‑6.22, P=0.019), dominant (del/ins+ins/ins vs. del/del; OR=2.19, 95% CI=1.20‑4.01, P=0.015) and allelic (ins vs. del; OR=1.90, 95% CI=1.25‑2.88, P=0.003) inheritance models. In summary, the findings propose a significant association between the VEGF 18‑bp ins/del polymorphism and risk of RSA in a sample of the southeast Iranian population. Further studies on larger sample sizes and different ethnicities are required to validate the present findings.

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