MicroRNA array analysis of the regulation of microRNAs in rats exhibiting hyperplasia of mammary glands

Gu,Liyan; Xu,Qingxin; Liu,Haitao; Xie,Jingru; Zhang,Lide

doi:10.3892/br.2018.1106

MicroRNA array analysis of the regulation of microRNAs in rats exhibiting hyperplasia of mammary glands

Authors:
- Liyan Gu
- Qingxin Xu
- Haitao Liu
- Jingru Xie
- Lide Zhang
View Affiliations

Affiliations: Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110847, P.R. China, Graduate School of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110847, P.R. China, The General Hospital of Chinese Armed Police Force, Beijing 100089, P.R. China, Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110847, P.R. China
Published online on: June 1, 2018 https://doi.org/10.3892/br.2018.1106
Pages: 142-146

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Hyperplasia of mammary glands (HMG) is also termed mammary dysplasia. In China, the number of patients suffering from breast hyperplasia is increasing annually. MicroRNAs (miRNAs; length, 19‑24 nucleotides), a group of small endogenous non‑coding RNAs, post‑transcriptionally regulate gene expression via RNA interference and gene silencing pathways. The cause of disease of HMG because remains unclear. Thus, the aim of the present study was to establish comprehensive profile of drug treatments following at different time intervals on rat models of differentially expressed miRNAs, using miRNA microarray data. After scanning the chip, 13 up‑regulated and 20 down‑regulated miRNAs were identified. MiR‑31 and miR‑30 exhibited different expression levels between rats exhibiting mammary gland hyperplasia treated with or without Jiedu Capsule water solution once a day for 4 weeks, and the two demonstrated a strong association with HMG and breast cancer. Therefore, the functions of these miRNAs may provide the basis for further investigation of HMG.

View Figures

View References

1	Chang XJ, Zhou J, Zhang S, Chen J, Chen CM, Wang ZZ and Xiao W: Effects of guizhi fuling capsule on sex hormone levels and breast issue morphology of mammary gland hyperplasia model rats. Zhongguo Zhong Yao Za Zhi. 39:4139–4142. 2014.(In Chinese). PubMed/NCBI
2	Liu B and Guan ZH: Radix bupleuri Extract Inhibits Hyperplasia of Mammary Gland in Rats. Trop J Pharm Res. 15:2932016. View Article : Google Scholar
3	Ekmekci C, Mousses S and Tuzmen S: MicroRNA (miRNA), involvement in aberrant promoter methylation facilitating tumor progression. Cancer Res. 67:1046. 2007.PubMed/NCBI
4	Rahimi G, Jafari N, Khodabakhsh M, Shirzad Z and Dogaheh HP: Upregulation of microRNA processing enzymes Drosha and Dicer in gestational diabetes mellitus. Gynecol Endocrinol. 31:156–159. 2015. View Article : Google Scholar : PubMed/NCBI
5	Mallick B and Ghosh Z: Probing Evolutionary Biography of MicroRNAs and Associated Factors. Curr Genomics. 13:144–152. 2012. View Article : Google Scholar : PubMed/NCBI
6	Bae S, Lee EM, Cha HJ, Kim K, Yoon Y, Lee H, Kim J, Kim YJ, Lee HG, Jeung HK, et al: Resveratrol alters microRNA expression profiles in A549 human non-small cell lung cancer cells. Mol Cells. 32:243–249. 2011. View Article : Google Scholar : PubMed/NCBI
7	Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI
8	Martins IJ: Induction of NAFLD with Increased Risk of Obesity and Chronic Diseases in Developed Countries. Open J Endocr Metab Dis. 04:90–110. 2014. View Article : Google Scholar
9	Badri H, Monsieurs P, Coninx I, Nauts R, Wattiez R and Leys N: Temporal Gene Expression of the Cyanobacterium Arthrospira in Response to Gamma Rays. PLoS One. 10:e01355652015. View Article : Google Scholar : PubMed/NCBI
10	Vyas S and Kumaranayake L: Constructing socio-economic status indices: How to use principal components analysis. Health Policy Plan. 21:459–468. 2006. View Article : Google Scholar : PubMed/NCBI
11	Lee C, Abdool A and Huang CH: PCA-based population structure inference with generic clustering algorithms. BMC Bioinformatics. 10(Suppl 1): S732009. View Article : Google Scholar : PubMed/NCBI
12	Venkatadri R, Muni T, Iyer AKV, Yakisich JS and Azad N: Role of apoptosis-related miRNAs in resveratrol-induced breast cancer cell death. Cell Death Dis. 7:e21042016. View Article : Google Scholar : PubMed/NCBI
13	Dupont WD and Page DL: Risk factors for breast cancer in women with proliferative breast disease. N Engl J Med. 312:146–151. 1985. View Article : Google Scholar : PubMed/NCBI
14	Dupont WD, Parl FF, Hartmann WH, Brinton LA, Winfield AC, Worrell JA, Schuyler PA and Plummer WD: Breast cancer risk associated with proliferative breast disease and atypical hyperplasia. Cancer. 71:1258–1265. 1993. View Article : Google Scholar : PubMed/NCBI
15	Cho JH, Dimri M and Dimri GP: MicroRNA-31 is a transcriptional target of histone deacetylase inhibitors and a regulator of cellular senescence. J Biol Chem. 290:10555–10567. 2015. View Article : Google Scholar : PubMed/NCBI
16	Rasheed SA, Teo CR, Beillard EJ, Voorhoeve PM, Zhou W, Ghosh S and Casey PJ: MicroRNA-31 controls G protein alpha-13 (GNA13) expression and cell invasion in breast cancer cells. Mol Cancer. 14:672015. View Article : Google Scholar : PubMed/NCBI
17	Valastyan S, Reinhardt F, Benaich N, Calogrias D, Szász AM, Wang ZC, Brock JE, Richardson AL and Weinberg RA: A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis. Cell. 137:1032–1046. 2009. View Article : Google Scholar : PubMed/NCBI
18	Cho JH, Dimri M and Dimri GP: MicroRNA-31 is a transcriptional target of histone deacetylase inhibitors and a regulator of cellular senescence. J Biol Chem. 290:10555–10567. 2015. View Article : Google Scholar : PubMed/NCBI
19	Ouzounova M, Vuong T, Ancey PB, Ferrand M, Durand G, Le-Calvez Kelm F, Croce C, Matar C, Herceg Z and Hernandez-Vargas H: MicroRNA miR-30 family regulates non-attachment growth of breast cancer cells. BMC Genomics. 14:1392013. View Article : Google Scholar : PubMed/NCBI
20	Bockhorn J, Dalton R, Nwachukwu C, Huang S, Prat A, Yee K, Chang YF, Huo D, Wen Y, Swanson KE, et al: MicroRNA-30c inhibits human breast tumour chemotherapy resistance by regulating TWF1 and IL-11. Nat Commun. 4:13932013. View Article : Google Scholar : PubMed/NCBI