Open Access

Mechanism of ginsenoside Rb3 against OGD/R damage based on metabonomic and PCR array analyses

  • Authors:
    • Fuhui Li
    • Jie Tao
    • Mingmin Zhou
    • Xingzhi Yu
    • Tian Xiao
    • Chaoliang Wang
    • Xiaohua Duan
  • View Affiliations

  • Published online on: October 9, 2024     https://doi.org/10.3892/br.2024.1875
  • Article Number: 187
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In order to study the mechanisms of ginsenoside Rb3 (G‑Rb3) against oxygen‑glucose deprivation/reoxygenation (OGD/R) injury in HT22 cells based on metabolomics and PCR array, HT22 cells were randomly divided into control group, model group, G‑Rb3 high‑dose group (10 µmol/l) and G‑Rb3 low‑dose group (5 µmol/l). Except for the control group, which was left untreated, the remaining groups were incubated with 10 mmol/l Na2S2O4 in sugar‑free DMEM medium for 2 h and then replaced with serum‑free high‑sugar DMEM medium for 2 h in order to replicate in vitro OGD/R model. Trypan blue staining was used to detect the cell viability; flow cytometry was used to detect apoptosis; western blotting was used to detect the protein expression levels of Bax, Bcl‑2 and caspase‑3. The metabolomics were used to analyze the differential metabolites of G‑Rb3 affecting OGD/R in order to find the relevant metabolic pathways. PCR array assay was performed to identify the expression of the differential genes. G‑Rb3 could inhibit HT22 apoptosis according to the result of cell morphology, trypan blue staining and flow cytometry. The levels of Bax and caspase‑3 protein expression were decreased, whereas the level of Bcl‑2 protein expression was increased after the treatment of G‑Rb3. Metabolomics results showed that a total of 31 differential metabolites between OGD/R group and G‑Rb3 group, such as guanosine level, was downregulated, the levels of enalaprilat and sorbitol were upregulated, affecting ABC transporters, galactose metabolism, citrate cycle and other related metabolic pathways; according to the result of PCR array, it was observed that G‑Rb3 significantly downregulated Trp63, Trp73, Dapk1, Casp14 and Cd70 pro‑apoptotic genes. In conclusion, G‑Rb3 has a significant protective effect on the OGD/R model simulated in vitro, and the mechanism may be related to the inhibition of apoptosis by affecting metabolites.
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Copy and paste a formatted citation
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Spandidos Publications style
Li F, Tao J, Zhou M, Yu X, Xiao T, Wang C and Duan X: Mechanism of ginsenoside Rb3 against OGD/R damage based on metabonomic and PCR array analyses. Biomed Rep 21: 187, 2024.
APA
Li, F., Tao, J., Zhou, M., Yu, X., Xiao, T., Wang, C., & Duan, X. (2024). Mechanism of ginsenoside Rb3 against OGD/R damage based on metabonomic and PCR array analyses. Biomedical Reports, 21, 187. https://doi.org/10.3892/br.2024.1875
MLA
Li, F., Tao, J., Zhou, M., Yu, X., Xiao, T., Wang, C., Duan, X."Mechanism of ginsenoside Rb3 against OGD/R damage based on metabonomic and PCR array analyses". Biomedical Reports 21.6 (2024): 187.
Chicago
Li, F., Tao, J., Zhou, M., Yu, X., Xiao, T., Wang, C., Duan, X."Mechanism of ginsenoside Rb3 against OGD/R damage based on metabonomic and PCR array analyses". Biomedical Reports 21, no. 6 (2024): 187. https://doi.org/10.3892/br.2024.1875