Open Access

Palmitoleate inhibits insulin transcription by activating the ERK1/2 pathway in rat pancreatic β-cells

  • Authors:
    • Yumei Yang
    • Liangliang Gong
  • View Affiliations

  • Published online on: April 18, 2017     https://doi.org/10.3892/etm.2017.4344
  • Pages: 2805-2811
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to evaluate the effects of palmitoleate on insulin secretion and insulin mRNA levels, and to investigate the transcriptional regulation of insulin. INS‑1 rat insulinoma cells were treated with palmitoleate in the presence of high glucose, and the amount of secreted insulin was measured via radioimmunoassay. Reverse transcription-quantitative polymerase chain reaction was performed to evaluate the mRNA levels of insulin and pancreatic and duodenal homeobox 1 (PDX1) under palmitoleate treatment. The levels of PDX1, peroxisome proliferator‑activated receptor gamma (PPARG), extracellular signal‑regulated kinase (ERK)1/2 and phosphorylated ERK1/2 were measured using western blot analysis. Low concentrations of palmitoleate significantly induced insulin secretion (P=0.024), whereas the mRNA levels of insulin and PDX1 were markedly reduced. However, the inhibitory effects were reversed with the addition of U0126, suggesting that the ERK1/2‑mediated pathway may be the underlying mechanism responsible for palmitoleate-induced downregulation of insulin mRNA. Exposure of INS‑1 cells to high glucose significantly increased the phosphorylation of ERK1/2 (P=0.039), which was further enhanced by palmitoleate (P=0.025). Exposure of INS‑1 cells to high glucose significantly decreased PPARG (P=0.001), which was further decreased by the addition of palmitoleate. U0126 was able to reverse the palmitoleate‑induced effects. In conclusion, the present study suggested that palmitoleate may induce insulin secretion and inhibit insulin mRNA expression in pancreatic β-cells.

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June-2017
Volume 13 Issue 6

Print ISSN: 1792-0981
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Spandidos Publications style
Yang Y and Gong L: Palmitoleate inhibits insulin transcription by activating the ERK1/2 pathway in rat pancreatic β-cells. Exp Ther Med 13: 2805-2811, 2017.
APA
Yang, Y., & Gong, L. (2017). Palmitoleate inhibits insulin transcription by activating the ERK1/2 pathway in rat pancreatic β-cells. Experimental and Therapeutic Medicine, 13, 2805-2811. https://doi.org/10.3892/etm.2017.4344
MLA
Yang, Y., Gong, L."Palmitoleate inhibits insulin transcription by activating the ERK1/2 pathway in rat pancreatic β-cells". Experimental and Therapeutic Medicine 13.6 (2017): 2805-2811.
Chicago
Yang, Y., Gong, L."Palmitoleate inhibits insulin transcription by activating the ERK1/2 pathway in rat pancreatic β-cells". Experimental and Therapeutic Medicine 13, no. 6 (2017): 2805-2811. https://doi.org/10.3892/etm.2017.4344