Open Access

Directly reprogramming fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by defined factors

  • Authors:
    • Wei Wu
    • Yu‑Qing Jin
    • Zhen Gao
  • View Affiliations

  • Published online on: April 20, 2017     https://doi.org/10.3892/etm.2017.4365
  • Pages:2685-2690
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

0

Abstract

The reprogramming of adult cells into pluripotent cells or directly into alternative adult cell types represents a great potential technology for regenerative medicine. In the present study, the potential of key developmental adipogenic, neurogenic and hepatogenic regulators to reprogram human fibroblasts into adipocytes, neurocytes and hepatocytes was investigated. The results demonstrated that direct reprogramming of octamer‑binding transcription factor 4 (Oct4) and CCAAT-enhancer-binding protein (C/EBP)β activated C/EBPα and peroxisome proliferator‑activated receptor‑γ expression, inducing the conversion of fibroblasts into adipocytes. Similarly, direct reprogramming of the transcription factors sex determining region‑box 2, trans‑acting T‑cell specific transcription factor (GATA‑3) and neurogenic differentiation 1 in fibroblasts may induce neurogenic differentiation through hemagglutinating virus of Japan envelope (HVJ‑E) transfection. Moreover, hepatogenic differentiation was induced by combining the direct reprogramming of Oct4, GATA‑3, hepatocyte nuclear factor 1 homeobox α and forkhead box protein A2 in fibroblasts. These results demonstrate that specific transcription factors and reprogramming factors are able to directly reprogram fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by HVJ‑E transfection.

Related Articles

Journal Cover

June 2017
Volume 13 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Wu, W., Jin, Y., & Gao, Z. (2017). Directly reprogramming fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by defined factors. Experimental and Therapeutic Medicine, 13, 2685-2690. https://doi.org/10.3892/etm.2017.4365
MLA
Wu, W., Jin, Y., Gao, Z."Directly reprogramming fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by defined factors". Experimental and Therapeutic Medicine 13.6 (2017): 2685-2690.
Chicago
Wu, W., Jin, Y., Gao, Z."Directly reprogramming fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by defined factors". Experimental and Therapeutic Medicine 13, no. 6 (2017): 2685-2690. https://doi.org/10.3892/etm.2017.4365