Open Access

Hyperbranched polymer drug delivery treatment for lung metastasis of salivary adenoid cystic carcinoma in nude mice

  • Authors:
    • Weiwei Wang
    • Jialei Ma
    • Furong Jin
    • Jianxing Liao
  • View Affiliations

  • Published online on: August 8, 2017     https://doi.org/10.3892/etm.2017.4902
  • Pages: 3105-3111
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Salivary adenoid cystic carcinoma (SACC) is associated with a high rate of lung metastasis. When lung metastasis occurs, the effects of traditional chemotherapy on SACC are poor. Hyperbranched polymer drug delivery (degradable hyperbranched polyglycerols, dHPGs) can be used as a strategy to load several drugs, and obtain beneficial effects on SACC lung metastasis through enhanced permeability and retention. In the present study, hydroxycamptothecin (HPT)‑conjugated dHPG (dHPG‑HPT) was synthesized and its effects on SACC xenografts in the lungs of nude mice were evaluated. SACC cells with a high potential for pulmonary metastasis (SACC‑LM cells) were injected into the tail vein of mice, establishing a nude mouse model. The mice were randomly divided into the three following groups: Control, HPT and dHPG‑HPT. Saline (control), HPT or dHPG‑HPT were injected into the mice. After two weeks, the mice were euthanized and their lungs were removed. The lungs were paraffin‑embedded for hematoxylin and eosin, and immunohistochemical staining analyses. Primary antibo­dies directed against vascular endothelial growth factor (VEGF), cluster of differentiation 34 (CD34), proliferating cell nuclear antigen (PCNA) and matrix metalloproteinase 9 (MMP9) were used. Gross observation demonstrated that the volumes of SACC lung metastasis nodules were significantly decreased in the dHPG‑HPT group compared with the control and HPT groups. Immunohistochemical analysis revealed a lower expression of VEGF, CD34, PCNA and MMP9 in the dHPG‑HPT group. The results of the current study suggest that dHPG‑HPT can suppress the growth of SACC xenografts in nude mice, providing a theoretical basis for macromolecular drug delivery‑based treatment of SACC.

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October-2017
Volume 14 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang W, Ma J, Jin F and Liao J: Hyperbranched polymer drug delivery treatment for lung metastasis of salivary adenoid cystic carcinoma in nude mice. Exp Ther Med 14: 3105-3111, 2017
APA
Wang, W., Ma, J., Jin, F., & Liao, J. (2017). Hyperbranched polymer drug delivery treatment for lung metastasis of salivary adenoid cystic carcinoma in nude mice. Experimental and Therapeutic Medicine, 14, 3105-3111. https://doi.org/10.3892/etm.2017.4902
MLA
Wang, W., Ma, J., Jin, F., Liao, J."Hyperbranched polymer drug delivery treatment for lung metastasis of salivary adenoid cystic carcinoma in nude mice". Experimental and Therapeutic Medicine 14.4 (2017): 3105-3111.
Chicago
Wang, W., Ma, J., Jin, F., Liao, J."Hyperbranched polymer drug delivery treatment for lung metastasis of salivary adenoid cystic carcinoma in nude mice". Experimental and Therapeutic Medicine 14, no. 4 (2017): 3105-3111. https://doi.org/10.3892/etm.2017.4902