MicroRNA‑191‑5p exerts a tumor suppressive role in renal cell carcinoma
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- Published online on: November 28, 2017 https://doi.org/10.3892/etm.2017.5581
- Pages: 1686-1693
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Abstract
Renal cell carcinoma (RCC) is a common tumor of the urinary system. Previously, miR‑191‑5p has been reported to be associated with various types of cancer; however, its specific functions in RCC have not been investigated to date. In the present study, the expression of miR‑191‑5p in the 786‑O and ACHN cell lines was detected in vitro by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The results of RT‑qPCR revealed that miR‑191‑5p was significantly downregulated in the two cell lines compared with the 293T cell line. miR‑191‑5p was also significantly downregulated in RCC tissue compared with paired normal tissue. In addition, the effects of miR‑191‑5p on cell proliferation, migration, invasion and apoptosis were examined by CCK‑8, MTT, wound scratch, Transwell and flow cytometry assays. Downregulation of miR‑191‑5p was observed to promote cell proliferation, migration and invasion, as well as to repress the cell apoptosis of 786‑O and ACHN cells. Therefore, the current study suggests that miR‑191‑5p functions as a tumor suppressor in RCC. Further studies are required to uncover the underlying signaling pathway of miR‑191‑5p and its potential role as a biomarker for early detection and prognosis prediction, and as a therapeutic target of RCC.
