Open Access

MicroRNA‑21‑5p expression in extracellular vesicles is increased in the blood of aging mice and in vascular endothelial cells induced by ionizing radiation

  • Authors:
    • Keisuke Yamamoto
    • Mitsuru Chiba
  • View Affiliations

  • Published online on: November 25, 2024     https://doi.org/10.3892/etm.2024.12772
  • Article Number: 22
  • Copyright: © Yamamoto et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In recent years, the Japanese population has been aging and the risk of contracting various age‑related diseases has increased. Thus, there is a need to analyze components that are characteristic of aging and examine their association with diseases to detect age‑related diseases at an early stage. In the present study, microRNAs (miRNAs/miRs) in serum extracellular vesicles (EVs) of 82‑102‑week‑old mice were analyzed to identify miRNAs characteristic of aging. Increased expression of mmu‑miR‑21a‑5p was observed. These miRNAs may be derived from senescent vascular endothelial cells, and RNA‑sequencing data (GSE130727) of HUVECs induced to senesce by 4 Gy of radiation revealed that the miRNAs were involved in the cell cycle and DNA repair. Annotations to senescence‑related pathways were also identified. Reduced expression of the miR‑21‑5p target gene, which has an identical sequence in humans and mice, was confirmed. In HUVECs induced to age under similar conditions, increased senescence‑associated β‑galactosidase activity and increased intracellular miR‑21‑5p expression were observed. A portion of the miR‑21‑5p was secreted extracellularly by internalizing tetraspanin‑positive EVs, and miR‑21‑5p was secreted into the extracellular space. The present study also demonstrated that miR‑21‑5p expression was upregulated and extracellular secretion of miR‑21‑5p was enhanced during vascular endothelial cell senescence. These findings suggested that increased serum miR‑21‑5p represents a biomarker for vascular endothelial cell senescence.
View Figures
View References

Related Articles

Journal Cover

February-2025
Volume 29 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yamamoto K and Chiba M: MicroRNA‑21‑5p expression in extracellular vesicles is increased in the blood of aging mice and in vascular endothelial cells induced by ionizing radiation. Exp Ther Med 29: 22, 2025.
APA
Yamamoto, K., & Chiba, M. (2025). MicroRNA‑21‑5p expression in extracellular vesicles is increased in the blood of aging mice and in vascular endothelial cells induced by ionizing radiation. Experimental and Therapeutic Medicine, 29, 22. https://doi.org/10.3892/etm.2024.12772
MLA
Yamamoto, K., Chiba, M."MicroRNA‑21‑5p expression in extracellular vesicles is increased in the blood of aging mice and in vascular endothelial cells induced by ionizing radiation". Experimental and Therapeutic Medicine 29.2 (2025): 22.
Chicago
Yamamoto, K., Chiba, M."MicroRNA‑21‑5p expression in extracellular vesicles is increased in the blood of aging mice and in vascular endothelial cells induced by ionizing radiation". Experimental and Therapeutic Medicine 29, no. 2 (2025): 22. https://doi.org/10.3892/etm.2024.12772