Enhancement of liposomal gene delivery in human breast cancer cells by dimethyl sulfoxide.

Jain,P T; Gewirtz,D A

doi:10.3892/ijmm.1.3.609

Mar 1998 Volume 1 Issue 3

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Mar 1998 Volume 1 Issue 3

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Article

Enhancement of liposomal gene delivery in human breast cancer cells by dimethyl sulfoxide.

Authors:
- P T Jain
- D A Gewirtz
View Affiliations / Copyright

Affiliations: Departments of Pharmacology, Toxicology and Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA.
Pages: 609-620
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Published online on: March 1, 1998

https://doi.org/10.3892/ijmm.1.3.609
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Abstract

Non-toxic concentrations ( 1%) of dimethyl sulfoxide (DMSO) enhance the liposomal delivery of DNA to both MCF-7 and MDA-MB-231 human breast tumor cells. Uptake of SV-40-luciferase was enhanced in MCF-7 cells by 14-fold while uptake of CMV-beta-galactosidase was increased 10-fold. In MDA-MB-231 cells, uptake of SV-40-luciferase was increased by approximately 70%. A mixture of ethanol and polyethylene glycol (45:55) at a concentration of 1% produced less pronounced improvements in transgene delivery to MCF-7 cells (a 70% increase in SV-40-luciferase uptake and a 4-fold increase in CMV-beta-galactosidase uptake) but no improvement in SV-40-luciferase gene delivery to MDA-MB-231 cells. These studies suggest that select pharmaceutical adjuvants which dissolve clinically useful drugs may have promise as non-toxic vehicles for improving transgene delivery. However, the relative effectiveness of these adjuvants is likely to vary depending on both the nature of the gene being delivered as well as the tumor cell which is the target for uptake of the exogenous gene.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Enhancement of liposomal gene delivery in human breast cancer cells by dimethyl sulfoxide.

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Abstract

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