Liprin-α2 gene, protein tyrosine phosphatase LAR interacting protein related gene, is downregulated by androgens in the human prostate cancer cell line LNCaP

  • Authors:
    • Kiyoshi Fujinami
    • Hiroji Uemura
    • Hitoshi Ishiguro
    • Yoshinobu Kubota
  • View Affiliations

  • Published online on: August 1, 2002     https://doi.org/10.3892/ijmm.10.2.173
  • Pages: 173-176
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Abstract

Prostate cancer is one of the most common neoplasms in the USA and Europe. We used differential display PCR (DD-PCR) to identify androgen-regulated genes in prostate cancer. The RNA of LNCaP cells treated with dihydrotestosterone (DHT) was analyzed for differentially expressed genes. Using DD-PCR, we identified a down-regulated cDNA fragment by DHT in LNCaP cells. This fragment was cloned and expressions of this fragment in prostate cancer cell lines were analyzed by RT-PCR. Sequence analysis revealed that a cDNA fragment is identical to protein tyrosine phosphatase LAR related gene, liprin-α2. liprin-α2 was downregulated by dihydrotestosterone (DHT) in LNCaP cells in a time- and androgen concentration-dependent manner. Downregulation by DHT was not inhibited by the protein synthesis inhibitor cycloheximide. This liprin-α2 gene was not expressed in androgen independent prostate cancer cell lines PC-3 and DU-145 at the mRNA level. And also, we first revealed here that liprin-α2 mRNA is expressed in LNCaP cells as well as human prostate cancer tissues and normal prostate tissues. These data suggest that liprin-α2 might play a role in androgen responsive human prostate cancer cell line as well as human prostate cells, and the loss of this gene expression might be associated with the androgen independent characteristics of prostate cancer.

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August 2002
Volume 10 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Fujinami K, Uemura H, Ishiguro H and Kubota Y: Liprin-α2 gene, protein tyrosine phosphatase LAR interacting protein related gene, is downregulated by androgens in the human prostate cancer cell line LNCaP. Int J Mol Med 10: 173-176, 2002.
APA
Fujinami, K., Uemura, H., Ishiguro, H., & Kubota, Y. (2002). Liprin-α2 gene, protein tyrosine phosphatase LAR interacting protein related gene, is downregulated by androgens in the human prostate cancer cell line LNCaP. International Journal of Molecular Medicine, 10, 173-176. https://doi.org/10.3892/ijmm.10.2.173
MLA
Fujinami, K., Uemura, H., Ishiguro, H., Kubota, Y."Liprin-α2 gene, protein tyrosine phosphatase LAR interacting protein related gene, is downregulated by androgens in the human prostate cancer cell line LNCaP". International Journal of Molecular Medicine 10.2 (2002): 173-176.
Chicago
Fujinami, K., Uemura, H., Ishiguro, H., Kubota, Y."Liprin-α2 gene, protein tyrosine phosphatase LAR interacting protein related gene, is downregulated by androgens in the human prostate cancer cell line LNCaP". International Journal of Molecular Medicine 10, no. 2 (2002): 173-176. https://doi.org/10.3892/ijmm.10.2.173