The PPARγ ligand, 15-Deoxy-Δ12,14-PGJ2, regulates apoptosis-related protein expression in cholangio cell carcinoma cells

  • Authors:
    • Hiroshi Okano
    • Katsuya Shiraki
    • Hidekazu Inoue
    • Tomoyuki Kawakita
    • Masatoshi Deguchi
    • Kazushi Sugimoto
    • Takahisa Sakai
    • Kazumoto Murata
    • Takeshi Nakano
    • Munechika Enjoji
  • View Affiliations

  • Published online on: December 1, 2003     https://doi.org/10.3892/ijmm.12.6.867
  • Pages: 867-870
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Abstract

PPARγ is known to induce apoptosis in malignant tumor cells, but the mechanism of this induction is not well understood. We investigated induction of apoptosis with 15-Deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2), a PPARγ ligand, in cholangio cell carcinoma (CCC) cells (RBE, ETK-1 or HuCCT-1). Apoptosis was induced in RBE and ETK-1 cells with 15d-PGJ2, but not in HuCCT-1 cells, although PPARγ was expressed in all CCC cells. Apoptosis-related proteins were also expressed, including FLIP, bclx, Apaf-1 and XIAP, but expression levels differed among the three cell lines. RBE cells treated with 15d-PGJ2 showed caspase activation, and it appeared that PPARγ-induced apoptosis was dependent on caspase activation. However, neither ETK-1 nor HuCCT-1 cells showed significant activation of caspase-8 or -3 with 15d-PGJ2 treatment, raising the possibility of a caspase-independent apoptosis induction pathway. XIAP was down-regulated by 15d-PGJ2 in all three CCC cell lines. Therefore, 15d-PGJ2 induces apoptosis in CCC cells via caspase-dependent or independent pathways. 15d-PGJ2 may also induce down-regulation of XIAP and may promote caspase cascade activation through TNF-family receptor signaling pathways.

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December 2003
Volume 12 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Okano H, Shiraki K, Inoue H, Kawakita T, Deguchi M, Sugimoto K, Sakai T, Murata K, Nakano T, Enjoji M, Enjoji M, et al: The PPARγ ligand, 15-Deoxy-Δ12,14-PGJ2, regulates apoptosis-related protein expression in cholangio cell carcinoma cells. Int J Mol Med 12: 867-870, 2003.
APA
Okano, H., Shiraki, K., Inoue, H., Kawakita, T., Deguchi, M., Sugimoto, K. ... Enjoji, M. (2003). The PPARγ ligand, 15-Deoxy-Δ12,14-PGJ2, regulates apoptosis-related protein expression in cholangio cell carcinoma cells. International Journal of Molecular Medicine, 12, 867-870. https://doi.org/10.3892/ijmm.12.6.867
MLA
Okano, H., Shiraki, K., Inoue, H., Kawakita, T., Deguchi, M., Sugimoto, K., Sakai, T., Murata, K., Nakano, T., Enjoji, M."The PPARγ ligand, 15-Deoxy-Δ12,14-PGJ2, regulates apoptosis-related protein expression in cholangio cell carcinoma cells". International Journal of Molecular Medicine 12.6 (2003): 867-870.
Chicago
Okano, H., Shiraki, K., Inoue, H., Kawakita, T., Deguchi, M., Sugimoto, K., Sakai, T., Murata, K., Nakano, T., Enjoji, M."The PPARγ ligand, 15-Deoxy-Δ12,14-PGJ2, regulates apoptosis-related protein expression in cholangio cell carcinoma cells". International Journal of Molecular Medicine 12, no. 6 (2003): 867-870. https://doi.org/10.3892/ijmm.12.6.867