search button

Lack of correlation between telomere length and telomerase activity and expression in leukemic cells

  • Authors:
    • Danuta Januszkiewicz
    • Jacek Wysoki
    • Krzysztof Lewandowski
    • Monika Pernak
    • Karina Nowicka
    • Jolanta Rembowska
    • Jerzy Nowak

  • View Affiliations

  • Published online on: December 1, 2003     https://doi.org/10.3892/ijmm.12.6.935
  • Pages: 935-938
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The expression of three components of telomerase complex (hTR, hTERT, TP1) along with telomerase activity and telomere length in leukemic cells was investigated. Cells were isolated from peripheral blood and/or bone marrow of children with acute lymphoblastic (ALL) and non-lymphoblastic (ANLL) leukemia. Expression of three components of telomerase as well as telomerase activity was found in all leukemic cells. Chemiluminescent detection of terminal restriction fragments (TRF) from DNA isolated from ALL cells showed variable patterns expressing considerable heterogeneity of telomere length. The ALL cells appeared to have both long and short telomere lengths, in contrast to normal peripheral lymphocytes, which produced limited pattern of TRF. The ANLL cells produced predominantly short telomere pattern despite high telomerase activity and expression. It can be concluded that high telomerase activity and expression in leukemic cells is not always correlated with long telomeres (TRF pattern).

Related Articles

Journal Cover

December 2003
Volume 12 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 5.7
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 12.3
  • CiteScore Rank: Q1: #28/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 1.022
  • SCImago Journal Rank (SJR): 1.167
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Januszkiewicz D, Wysoki J, Lewandowski K, Pernak M, Nowicka K, Rembowska J and Nowak J: Lack of correlation between telomere length and telomerase activity and expression in leukemic cells. Int J Mol Med 12: 935-938, 2003.
APA
Januszkiewicz, D., Wysoki, J., Lewandowski, K., Pernak, M., Nowicka, K., Rembowska, J., & Nowak, J. (2003). Lack of correlation between telomere length and telomerase activity and expression in leukemic cells. International Journal of Molecular Medicine, 12, 935-938. https://doi.org/10.3892/ijmm.12.6.935
MLA
Januszkiewicz, D., Wysoki, J., Lewandowski, K., Pernak, M., Nowicka, K., Rembowska, J., Nowak, J."Lack of correlation between telomere length and telomerase activity and expression in leukemic cells". International Journal of Molecular Medicine 12.6 (2003): 935-938.
Chicago
Januszkiewicz, D., Wysoki, J., Lewandowski, K., Pernak, M., Nowicka, K., Rembowska, J., Nowak, J."Lack of correlation between telomere length and telomerase activity and expression in leukemic cells". International Journal of Molecular Medicine 12, no. 6 (2003): 935-938. https://doi.org/10.3892/ijmm.12.6.935