Role of caspase in CD80 expression of superantigen-stimulated monocytes
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- Published online on: August 1, 2004 https://doi.org/10.3892/ijmm.14.2.241
- Pages: 241-246
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Abstract
In this study, we demonstrated evidence for the induction of CD80+ monocytes by staphylococcal enterotoxin B (SEB) via caspase actions. Pre-treatment with pyrrolidine dithiocarbamate (PDTC), a potent inhibitor of NF-κB, resulted in a significant reduction in the percentage of SEB- and interferon-γ (IFN-γ) (produced by SEB) -induced CD80+ monocytes. SEB and IFN-γ activated NF-κB, which was inhibited by PDTC. SEB induced the activation of caspase-3 and -8, and pre-treatment with z-VAD-fmk, a broad-spectrum inhibitor of caspases, prevented the induction of apoptosis. Treating with z-VAD-fmk resulted in a reduction of the generation of CD80+ monocytes. These results indicated that CD80 driven by NF-κB is regulated by the enzymatic actions of caspases, which allows monocytes to participate in massive T-cell activation.
