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Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes

  • Authors:
    • Constanze Karst
    • Vladimir Trifonov
    • Svetlana A. Romanenko
    • Uwe Claussen
    • Kristin Mrasek
    • Susanne Michel
    • Philip Avner
    • Thomas Liehr

  • View Affiliations

  • Published online on: February 1, 2006     https://doi.org/10.3892/ijmm.17.2.209
  • Pages: 209-213
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Abstract

The Moloney murine leukemia virus-transformed suspension cell line WMP2 is derived from wild mice (Mus musculus) of the WMP/WMP strain. These mice carry nine pairs of metacentric Robertsonian translocation chromosomes. As the chromosomes of the wild-type mouse are all acrocentric, metaphase spreads of the WMP2 cells seam to be highly suited for physical gene mapping. Here we studied the WMP2 line using spectral karyotyping (SKY) combined with new established mouse specific multicolor banding (mcb) probes for the chromosomes X, 3, 4, 6 and 18. SKY revealed that the WMP2 cell line developed further four derivative chromosomes. After application of mcb five previously unrecognizable intrachromosomal rearrangements with 9 breakpoints were detected for the studied chromosomes.

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Journal Cover

February 2006
Volume 17 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • Journal Metrics
  • Impact Factor: 5.7
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 12.3
  • CiteScore Rank: Q1: #28/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 1.022
  • SCImago Journal Rank (SJR): 1.167
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Spandidos Publications style
Karst C, Trifonov V, Romanenko SA, Claussen U, Mrasek K, Michel S, Avner P and Liehr T: Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes. Int J Mol Med 17: 209-213, 2006.
APA
Karst, C., Trifonov, V., Romanenko, S.A., Claussen, U., Mrasek, K., Michel, S. ... Liehr, T. (2006). Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes. International Journal of Molecular Medicine, 17, 209-213. https://doi.org/10.3892/ijmm.17.2.209
MLA
Karst, C., Trifonov, V., Romanenko, S. A., Claussen, U., Mrasek, K., Michel, S., Avner, P., Liehr, T."Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes". International Journal of Molecular Medicine 17.2 (2006): 209-213.
Chicago
Karst, C., Trifonov, V., Romanenko, S. A., Claussen, U., Mrasek, K., Michel, S., Avner, P., Liehr, T."Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes". International Journal of Molecular Medicine 17, no. 2 (2006): 209-213. https://doi.org/10.3892/ijmm.17.2.209