Apolipoprotein-E modulates the cytotoxic effect of β-amyloid on rat brain endothelium in an isoform-dependent specific manner
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- Published online on: May 1, 2006 https://doi.org/10.3892/ijmm.17.5.821
- Pages: 821-826
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Abstract
Several studies support the hypothesis that apolipoprotein-E (ApoE) acts as a pathological chaperone protein that promotes the β-plated sheet conformation of β-amyloid (Aβ) peptides into amyloid fibers. In vitro evidence is also available that ApoE inhibits the neurotoxic effect of Aβ in an allele-specific manner (E2 ≥ E3 > E4). We have recently shown that Aβ peptides exert a time- and concentration-dependent toxic effect on rat neuromicrovascular endothelial cells (NECs), and this study aimed to ascertain whether ApoE isoforms are able to modulate this effect. ApoE2 and ApoE4 decreased and increased, respectively, the cytotoxic effect of Aβ(1-40) and Aβ(1-42) on NECs, as evaluated by their survival and viability rates. The toxic effect of both Aβ peptides and ApoE4 was associated with the rise in the necrosis rate of NECs within a 24-h incubation period. Moreover, ApoE2 prevented and ApoE4 magnified the inhibitory effect of Aβ on the capability of NECs cultured on Matrigel to form a capillary-like network. The opposite effects of ApoE isoforms could be due to their different interactions with the C-terminal domain of Aβ. ApoE2, at variance with ApoE4, is thought to form sodium dodecyl sulphate-stable complexes with Aβ and, as a consequence, it could block the interactions of the non-fibrillar Aβ peptide with the plasma membrane, Aβ peptide aggregation and the ensuing cytotoxicity. Collectively, our findings confirm the view that ApoE plays a relevant role in the pathogenesis of Alzheimer's disease.