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Perturbation of glycerol metabolism in hepatocytes from n3-PUFA-depleted rats

  • Authors:
    • Laurence Portois
    • Ying Zhang
    • Laurence Ladrière
    • Jason Perret
    • Karim Louchami
    • Nathalie Gaspard
    • Emeline Hupkens
    • Nargis Bolaky
    • Valérie Delforge
    • Renaud Beauwens
    • Willy J. Malaisse
    • Abdullah Sener
    • Yvon A. Carpentier
    • Christine Delporte

  • View Affiliations

  • Published online on: March 16, 2012     https://doi.org/10.3892/ijmm.2012.943
  • Pages: 1121-1126
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

Second generation n3-PUFA-depleted rats represent a good animal model of metabolic syndrome as they display several features of the disease such as liver steatosis, visceral obesity and insulin resistance. The goal of our study was to investigate the influence of n3-PUFA deficiency on hepatic glycerol metabolism. Aquaglyceroporin 9 (AQP9) allows hepatic glycerol transport and consequently contributes to neoglucogenesis. AQP9 knockout mice display hypertriacyl-glycerolemia, one of the hallmarks of the metabolic syndrome. Our data show reduced AQP9 expression at the protein level in n3-PUFA-depleted rats, without any changes at the mRNA levels. [U-14C]glycerol uptake was increased in hepatocytes from n3-PUFA-depleted animal cells. The apparent discrepancy between decreased AQP9 protein expression, and increased [U-14C]glycerol uptake could be explained by an observed increase in glycerol kinase activity.

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Journal Cover

June 2012
Volume 29 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • Journal Metrics
  • Impact Factor: 5.7
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 12.3
  • CiteScore Rank: Q1: #28/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 1.022
  • SCImago Journal Rank (SJR): 1.167
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Spandidos Publications style
Portois L, Zhang Y, Ladrière L, Perret J, Louchami K, Gaspard N, Hupkens E, Bolaky N, Delforge V, Beauwens R, Beauwens R, et al: Perturbation of glycerol metabolism in hepatocytes from n3-PUFA-depleted rats. Int J Mol Med 29: 1121-1126, 2012.
APA
Portois, L., Zhang, Y., Ladrière, L., Perret, J., Louchami, K., Gaspard, N. ... Delporte, C. (2012). Perturbation of glycerol metabolism in hepatocytes from n3-PUFA-depleted rats. International Journal of Molecular Medicine, 29, 1121-1126. https://doi.org/10.3892/ijmm.2012.943
MLA
Portois, L., Zhang, Y., Ladrière, L., Perret, J., Louchami, K., Gaspard, N., Hupkens, E., Bolaky, N., Delforge, V., Beauwens, R., Malaisse, W. ., Sener, A., Carpentier, Y. A., Delporte, C."Perturbation of glycerol metabolism in hepatocytes from n3-PUFA-depleted rats". International Journal of Molecular Medicine 29.6 (2012): 1121-1126.
Chicago
Portois, L., Zhang, Y., Ladrière, L., Perret, J., Louchami, K., Gaspard, N., Hupkens, E., Bolaky, N., Delforge, V., Beauwens, R., Malaisse, W. ., Sener, A., Carpentier, Y. A., Delporte, C."Perturbation of glycerol metabolism in hepatocytes from n3-PUFA-depleted rats". International Journal of Molecular Medicine 29, no. 6 (2012): 1121-1126. https://doi.org/10.3892/ijmm.2012.943