Open Access

Detection of lower levels of SNAP25 using multiple microarray systems and its functional significance in medulloblastoma

  • Authors:
    • Chi-Jung Huang
    • Chia-Long Lee
    • Chih-Yi Liu
    • Shih-Hung Huang
    • Jia-Woei Hou
    • Yi-Hou Chen
    • Chih-Cheng Chien
    • Chih-Ming Ho
    • Wen-Cheng Lo
    • Kun-Long Hung
  • View Affiliations

  • Published online on: March 20, 2017     https://doi.org/10.3892/ijmm.2017.2925
  • Pages:1195-1205
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Medulloblastoma (MB) is the most common pediatric malignant brain tumor and patients with high-risk or recurrent MB respond poorly to current therapies, and have a higher related mortality. For this reason, potential molecules related to MB need be identified in order to develop targets for the development of novel therapeutics. In the present study, we compared MB microarray data obtained using different microarray systems and significant targets were selected by gene annotation and enrichment analysis. Genes for soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) annotated with the function ‘vesicle’ were identified and one of these proteins, synaptosomal-associated protein 25 (SNAP25), was found to have significantly lower expression levels in MB. In addition, SNAP25 was detected in a very low number of MB cells as shown by western blot analysis and immunohistochemical analyses of archived and formalin-fixed/paraffin-embedded human MB specimens. We found that SNAP25 altered the morphology and the chemotherapeutic effects of arabinofuranosyl cytidine (Ara-C) on SNAP25-expressing MB cells. On the whole, our data indicate that the expression of SNAP25 is crucial for dendrite formation and is associated with the effects of targeted chemotherapy. The detection of SNAP25 expression in MB cells may thus be essential for the chemotherapeutic application of Ara-C.

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May 2017
Volume 39 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

2015 Impact Factor: 2.348
Ranked #22/123 Medicine Research and Experimental
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APA
Huang, C., Lee, C., Liu, C., Huang, S., Hou, J., Chen, Y. ... Hung, K. (2017). Detection of lower levels of SNAP25 using multiple microarray systems and its functional significance in medulloblastoma. International Journal of Molecular Medicine, 39, 1195-1205. https://doi.org/10.3892/ijmm.2017.2925
MLA
Huang, C., Lee, C., Liu, C., Huang, S., Hou, J., Chen, Y., Chien, C., Ho, C., Lo, W., Hung, K."Detection of lower levels of SNAP25 using multiple microarray systems and its functional significance in medulloblastoma". International Journal of Molecular Medicine 39.5 (2017): 1195-1205.
Chicago
Huang, C., Lee, C., Liu, C., Huang, S., Hou, J., Chen, Y., Chien, C., Ho, C., Lo, W., Hung, K."Detection of lower levels of SNAP25 using multiple microarray systems and its functional significance in medulloblastoma". International Journal of Molecular Medicine 39, no. 5 (2017): 1195-1205. https://doi.org/10.3892/ijmm.2017.2925