Black rice (Oryza sativa L.) extract modulates ultraviolet-induced expression of matrix metalloproteinases and procollagen in a skin cell model

Han,Mira; Bae,Jung‑Soo; Ban,Jae‑Jun; Shin,Hee  Soon; Lee,Dong  Hun; Chung,Jin  Ho

doi:10.3892/ijmm.2018.3508

Black rice (Oryza sativa L.) extract modulates ultraviolet-induced expression of matrix metalloproteinases and procollagen in a skin cell model

Authors:
- Mira Han
- Jung‑Soo Bae
- Jae‑Jun Ban
- Hee Soon Shin
- Dong Hun Lee
- Jin Ho Chung
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Affiliations: Department of Biomedical Sciences, Seoul National University Graduate School, Seoul 110‑744, Republic of Korea, Department of Dermatology, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea, Korea Food Research Institute, Seongnam‑si, Gyeonggi‑do 463‑746, Republic of Korea
Published online on: February 22, 2018 https://doi.org/10.3892/ijmm.2018.3508
Pages: 3073-3080

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Abstract

Exposure of the skin to ultraviolet (UV) radiation causes extracellular matrix (ECM) collapse in the dermis, owing to an increase in matrix metalloproteinase (MMP) production in both the epidermis and dermis, and a decrease in type I collagen expression in the dermis. Recently, black rice (Oryza sativa L.) was reported to have a wide range of pharmacological effects in various settings. However, the effects of black rice extract (BRE) on UV‑irradiated skin cells have not yet been characterized. BRE treatment did not affect cell morphology and viability of HaCaT and human dermal fibroblasts (HDF). We demonstrated that BRE downregulated basal and UV‑induced MMP‑1 expression in HaCaT cells. Furthermore, BRE significantly increased type I procollagen expression, and decreased MMP‑1 and MMP‑3 expression in UV‑irradiated HDF. The underlying mechanisms of these results involve a decrease in p38 and c‑Jun N‑terminal kinase activity, and suppression of UV‑induced activation of activator protein‑1 (AP‑1). BRE reduced UV‑induced reactive oxygen species production in HaCaT cells in a dose‑dependent manner. Indeed, mass spectrometry revealed that BRE contained antioxidative flavonoid components such as cyanidin‑3‑O‑β‑D‑glycoside and taxifolin‑7‑O‑glucoside. These findings suggest that BRE attenuates UV‑induced ECM damage by modulating mitogen‑activated protein kinase and AP‑1 signaling, and could be used as an active ingredient for preventing photoaging of the skin.

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