Impact of 3D-culture on the expression of differentiation markers and hormone receptors in growth plate chondrocytes as compared to articular chondrocytes

  • Authors:
    • Christian Albrecht
    • Magda Helmreich
    • Brigitte Tichy
    • Stefan Marlovits
    • Roberto Plasenzotti
    • Monika Egerbacher
    • Gabriele Haeusler
  • View Affiliations

  • Published online on: March 1, 2009     https://doi.org/10.3892/ijmm_00000138
  • Pages: 347-355
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Abstract

In this study we compared the expression of hormone receptors and markers of differentiation in growth plate chondrocytes (GPC) and articular chondrocytes (AC) under different culture conditions by real-time PCR and immunofluorescence. After one week of monolayer culture, porcine GPC and AC of 6-8-week-old piglets were either seeded in alginate beads or further grown in monolayer culture up to 5 weeks. During monolayer culture the expression of Col2, Col1, Col10, aggrecan, ERα and ERβ decreased dramatically, whereas culture in alginate beads restored an expression pattern similar to native tissue. The receptors IGF-1R, IGF-2R and GHR were, however, increased in monolayer culture compared to alginate culture or native tissue. The relative proportion of ERα and ERβ expression was different in GPC when compared to AC. All of our results on mRNA expression during culture in alginate beads were also verified to be translated to the protein level. Thus, the expression of hormone rereptors and differentiation markers were found to be highly influenced by culture conditions. Moreover, the porcine model is promising as defined by tissue availability, expression of relevant hormonal receptors and comparability to human conditions, in particular in the area of basic growth research.

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March 2009
Volume 23 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Albrecht C, Helmreich M, Tichy B, Marlovits S, Plasenzotti R, Egerbacher M and Haeusler G: Impact of 3D-culture on the expression of differentiation markers and hormone receptors in growth plate chondrocytes as compared to articular chondrocytes. Int J Mol Med 23: 347-355, 2009.
APA
Albrecht, C., Helmreich, M., Tichy, B., Marlovits, S., Plasenzotti, R., Egerbacher, M., & Haeusler, G. (2009). Impact of 3D-culture on the expression of differentiation markers and hormone receptors in growth plate chondrocytes as compared to articular chondrocytes. International Journal of Molecular Medicine, 23, 347-355. https://doi.org/10.3892/ijmm_00000138
MLA
Albrecht, C., Helmreich, M., Tichy, B., Marlovits, S., Plasenzotti, R., Egerbacher, M., Haeusler, G."Impact of 3D-culture on the expression of differentiation markers and hormone receptors in growth plate chondrocytes as compared to articular chondrocytes". International Journal of Molecular Medicine 23.3 (2009): 347-355.
Chicago
Albrecht, C., Helmreich, M., Tichy, B., Marlovits, S., Plasenzotti, R., Egerbacher, M., Haeusler, G."Impact of 3D-culture on the expression of differentiation markers and hormone receptors in growth plate chondrocytes as compared to articular chondrocytes". International Journal of Molecular Medicine 23, no. 3 (2009): 347-355. https://doi.org/10.3892/ijmm_00000138