Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening

  • Authors:
    • M. Gnanasekar
    • F. G. Suleman
    • K. Ramaswamy
    • J. D. Caldwell
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/ijmm_00000248
  • Pages: 421-426
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Abstract

The present study reports the identification of human sex hormone binding globulin (SHBG)-interacting proteins in the brain using a phage display-based screening technology. Phage display is a system in which a foreign protein is displayed on the surface of a bacteriophage as a fusion protein with one of the coat proteins of the bacteriophage. T7 phage clones expressing normal human brain proteins (human normal brain phage-display cDNA expression library) were screened using SHBG as bait. The bound phage clones were then identified by DNA sequencing and by BLAST search analysis. Of the twenty binding proteins analyzed, three were found to be membrane-associated proteins: synaptosomal associated protein 25 (SNAP25), Thy-1 cell surface antigen and zonadhesin. Further studies will determine if the interactions of SHBG with these proteins have any role in the internalization and cell signaling events or whether they contribute to steroid delivery to specific cells.

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October 2009
Volume 24 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Gnanasekar M, Suleman FG, Ramaswamy K and Caldwell JD: Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening. Int J Mol Med 24: 421-426, 2009.
APA
Gnanasekar, M., Suleman, F.G., Ramaswamy, K., & Caldwell, J.D. (2009). Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening. International Journal of Molecular Medicine, 24, 421-426. https://doi.org/10.3892/ijmm_00000248
MLA
Gnanasekar, M., Suleman, F. G., Ramaswamy, K., Caldwell, J. D."Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening". International Journal of Molecular Medicine 24.4 (2009): 421-426.
Chicago
Gnanasekar, M., Suleman, F. G., Ramaswamy, K., Caldwell, J. D."Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening". International Journal of Molecular Medicine 24, no. 4 (2009): 421-426. https://doi.org/10.3892/ijmm_00000248