The bone anabolic carotenoid β-cryptoxanthin enhances transforming growth factor-β1-induced SMAD activation in MC3T3 preosteoblasts

  • Authors:
    • Masayoshi Yamaguchi
    • M. Neale Weitzmann
  • View Affiliations

  • Published online on: November 1, 2009     https://doi.org/10.3892/ijmm_00000278
  • Pages: 671-675
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Abstract

The xanthophyll β-cryptoxanthin is a member of the carotenoid family of plant-derived pigments endowed with anti-osteoporotic properties in vivo. β-cryptoxanthin was demonstrated to stimulate osteoblastic bone formation and simultaneously repress osteoclastic bone resorption in vitro. However, the mechanisms of action remain to be elucidated. The SMAD signal transduction pathway is established to play a critical role in osteoblast lineage commitment and differentiation. In this study we used transient transfection assays of a SMAD luciferase reporter to investigate whether β-cryptoxanthin regulates SMAD activation in MC3T3 pre-osteoblastic cells. β-cryptoxanthin did not stimulate basal SMAD activity but amplified transforming growth factor (TGF)-β1-induced SMAD activation. Interestingly, β-cryptoxanthin did not affect bone morphogenetic protein-2 (BMP-2)-induced SMAD activation in osteoblastic cells, suggesting specificity of action on the TGF-β1 pathway. This study suggests that the carotenoid β-cryptoxanthin may promote osteoblast differentiation and activity by amplifying TGF-β1-induced lineage commitment of osteoblast precursors.

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November 2009
Volume 24 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yamaguchi M and Weitzmann MN: The bone anabolic carotenoid β-cryptoxanthin enhances transforming growth factor-β1-induced SMAD activation in MC3T3 preosteoblasts. Int J Mol Med 24: 671-675, 2009.
APA
Yamaguchi, M., & Weitzmann, M.N. (2009). The bone anabolic carotenoid β-cryptoxanthin enhances transforming growth factor-β1-induced SMAD activation in MC3T3 preosteoblasts. International Journal of Molecular Medicine, 24, 671-675. https://doi.org/10.3892/ijmm_00000278
MLA
Yamaguchi, M., Weitzmann, M. N."The bone anabolic carotenoid β-cryptoxanthin enhances transforming growth factor-β1-induced SMAD activation in MC3T3 preosteoblasts". International Journal of Molecular Medicine 24.5 (2009): 671-675.
Chicago
Yamaguchi, M., Weitzmann, M. N."The bone anabolic carotenoid β-cryptoxanthin enhances transforming growth factor-β1-induced SMAD activation in MC3T3 preosteoblasts". International Journal of Molecular Medicine 24, no. 5 (2009): 671-675. https://doi.org/10.3892/ijmm_00000278