Anti-carcinogenic effect of a new analogue 4'-chloroflavanone from flavanone in human breast cancer cells

  • Authors:
    • Eun Jeong Choi
    • Jae In Lee
    • Gun-Hee Kim
  • View Affiliations

  • Published online on: February 1, 2010     https://doi.org/10.3892/ijmm_00000344
  • Pages: 293-298
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Abstract

We investigated the antiproliferative effects of synthetic flavanone derivatives using an MTT assay in MCF-7 and MDA-MB-453 cells. When cells were treated with synthetic flavanone derivatives in concentrations ranging from 1 to 200 µM for 48 h, cell growth decreased at concentrations >50 µM. 4'-Chloroflavanone is more potent than flavanone among the synthetic flavanone derivatives. Exposure to 4'-chloroflavanone at 50 µM for 48 h caused cell cycle arrest in both MCF-7 and MDA-MB-453 cells. In addition, when 4'-chloroflavanone caused G1/S phase arrest, a decrease in CDK4 and cyclin D, together with an increase in p21Cip1, was observed in the cells. The p21Cip1 is a downstream target of p53 that may be affected by the activation of p53 by 4'-chloroflavanone. These results indicate that activation of p53 played some role in 4'-chloroflavanone-induced cell cycle arrest of human breast cancer cells. 4'-Chloroflavanone increased cytochrome c expression and decreased the expression of caspase-3, but did not change the expression of Bcl-2 and Bax. Activation of cytochrome c and its downstream target, caspase-3, is suggested to be an important inducer of the apoptosis process by 4'-chloroflavanone. 4'-Chloroflavanone inhibits cell proliferation through G1/S phase disruption and may induce apoptosis. Based on our findings, we propose that 4'-chloro-flavanone is useful as an anticancer drug.

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February 2010
Volume 25 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Choi EJ, Lee JI and Kim G: Anti-carcinogenic effect of a new analogue 4'-chloroflavanone from flavanone in human breast cancer cells. Int J Mol Med 25: 293-298, 2010.
APA
Choi, E.J., Lee, J.I., & Kim, G. (2010). Anti-carcinogenic effect of a new analogue 4'-chloroflavanone from flavanone in human breast cancer cells. International Journal of Molecular Medicine, 25, 293-298. https://doi.org/10.3892/ijmm_00000344
MLA
Choi, E. J., Lee, J. I., Kim, G."Anti-carcinogenic effect of a new analogue 4'-chloroflavanone from flavanone in human breast cancer cells". International Journal of Molecular Medicine 25.2 (2010): 293-298.
Chicago
Choi, E. J., Lee, J. I., Kim, G."Anti-carcinogenic effect of a new analogue 4'-chloroflavanone from flavanone in human breast cancer cells". International Journal of Molecular Medicine 25, no. 2 (2010): 293-298. https://doi.org/10.3892/ijmm_00000344