PROLIFERATIVE ACTIVITY IN ADVANCED GASTRIC-CANCER WITH KI-67 AND PROPIDIUM IODIDE - ANALYSIS BY FLOW-CYTOMETRY
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- Published online on: August 1, 1992 https://doi.org/10.3892/ijo.1.3.265
- Pages: 265-269
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Abstract
By setting the cut-off line at the level as used in a negative control study without primary antibody in the same sample, the Ki-67 labeling rate was calculated by the Ki67-DNA dual fluorescence analysis. The mean Ki-67 labeling rate of 28 advanced gastric cancer was 45.1% (13.9-76.3%). The Ki-67 labeling rates were significantly higher for larger size tumor (p<0.05), peritoneal metastasis and advanced stage (p<0.01). This is the first report that cycling cells are calculated by setting the cut-off line for solid tumors. Use of this flow cytometric procedure substantially facilitates the quantification of proliferating cells in gastric cancer.
