Multidrug resistance mediated by overexpression of P-glycoprotein in human osteosarcoma in vivo.

Suto,R; Abe,Y; Nakamura,M; Ohnishi,Y; Yoshimura,M; Lee,Y H; Imanishi,T; Yamazaki,H; Kijima,H; Tokunaga,T; Oshika,Y; Hiraoka,N; Tamaoki,N; Fukuda,H; Ueyama,Y

doi:10.3892/ijo.12.2.287

Multidrug resistance mediated by overexpression of P-glycoprotein in human osteosarcoma in vivo.

Authors:
- R Suto
- Y Abe
- M Nakamura
- Y Ohnishi
- M Yoshimura
- Y H Lee
- T Imanishi
- H Yamazaki
- H Kijima
- T Tokunaga
- Y Oshika
- N Hiraoka
- N Tamaoki
- H Fukuda
- Y Ueyama
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Affiliations: Department of Orthopedics, Tokai University, School of Medicine, Bohseidai, Isehara-shi, Kanagawa 259-11, Japan.
Published online on: February 1, 1998 https://doi.org/10.3892/ijo.12.2.287
Pages: 287-378

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Abstract

We examined the expression levels of P-glycoprotein (P-Gp)/the human multidrug resistance gene (MDR1) and in vivo chemosensitivity in the 7 osteosarcoma xenografts. Three of seven (43%) osteosarcoma xenografts expressed MDR1 by reverse transcriptase-polymerase chain reaction (RT-PCR). The OSS-516R xenograft selected with vincristine (VCR) from the MDR1-negative xenograft OSS-516, which was sensitive to VCR and doxorubicin (DOX), acquired cross-resistance to DOX. In the OSS-516R, RT-PCR assay showed definite MDR1 expression and immunohistochemical analysis demonstrated P-Gp-positive tumor cells. These results suggest that P-Gp/MDR1 overexpression is related to multidrug resistance in human osteosarcoma in vivo.