TGF-beta1 produced by gastric cancer cells affects mesothelial cell morphology in peritoneal dissemination.

  • Authors:
    • S Nishimura
    • K Y Hirakawa-Chung
    • M Yashiro
    • T Inoue
    • T Matsuoka
    • T Fujihara
    • K Murahashi
    • T Sawada
    • B Nakata
    • I Jikihara
    • H Takagi
    • M Sowa
  • View Affiliations

  • Published online on: April 1, 1998     https://doi.org/10.3892/ijo.12.4.847
  • Pages: 847-898
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Abstract

In vitro morphologic change of mesothelial cells was observed following the addition of serum-free conditioned medium (SF-CM) from peritoneal dissemination cell line OCUM-2MD3. The same morphologic change of mesothelial cells was observed following the addition of 10 ng/ml TGF-beta1, but not following the addition of b-FGF, IGF-I, VEGF or PDGF-AA. In the in vivo study, mesothelial cells of mice treated with SF-CM from OCUM-2MD3 and TGF- beta1 were separated from one another, resulting in exposure of the submesothelial connective tissue. The molecular size of the mesothelial morphology changing activity was estimated by running the SF-CM from OCUM-2MD3 through a gel filtration column TSK-gel G2000SW. The mesothelial morphology changing activity was recognized at positions equivalent of Mr 6, 500-30,000. 25 kDa TGF-beta1 was detected in the active fraction from the TSK-gel G2000SW column and the SF-CM of OCUM-2MD3 by Western blotting using a monoclonal antibody against TGF-beta1. These findings suggest that TGF-beta1 produced by gastric cancer cells changes the morphology of mesothelial cells and may thus be closely associated with peritoneal dissemination.

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Apr 1998
Volume 12 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Nishimura S, Hirakawa-Chung K, Yashiro M, Inoue T, Matsuoka T, Fujihara T, Murahashi K, Sawada T, Nakata B, Jikihara I, Jikihara I, et al: TGF-beta1 produced by gastric cancer cells affects mesothelial cell morphology in peritoneal dissemination.. Int J Oncol 12: 847-898, 1998.
APA
Nishimura, S., Hirakawa-Chung, K., Yashiro, M., Inoue, T., Matsuoka, T., Fujihara, T. ... Sowa, M. (1998). TGF-beta1 produced by gastric cancer cells affects mesothelial cell morphology in peritoneal dissemination.. International Journal of Oncology, 12, 847-898. https://doi.org/10.3892/ijo.12.4.847
MLA
Nishimura, S., Hirakawa-Chung, K., Yashiro, M., Inoue, T., Matsuoka, T., Fujihara, T., Murahashi, K., Sawada, T., Nakata, B., Jikihara, I., Takagi, H., Sowa, M."TGF-beta1 produced by gastric cancer cells affects mesothelial cell morphology in peritoneal dissemination.". International Journal of Oncology 12.4 (1998): 847-898.
Chicago
Nishimura, S., Hirakawa-Chung, K., Yashiro, M., Inoue, T., Matsuoka, T., Fujihara, T., Murahashi, K., Sawada, T., Nakata, B., Jikihara, I., Takagi, H., Sowa, M."TGF-beta1 produced by gastric cancer cells affects mesothelial cell morphology in peritoneal dissemination.". International Journal of Oncology 12, no. 4 (1998): 847-898. https://doi.org/10.3892/ijo.12.4.847