A COMPARATIVE-ANALYSIS OF THE ACTIVITY OF RETINOIC ACID AND CYCLIC-AMP IN THE INDUCTION OF DIFFERENTIATION OF EMBRYONAL CARCINOMA-CELLS
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- Published online on: March 1, 1993 https://doi.org/10.3892/ijo.2.3.403
- Pages: 403-412
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Abstract
Retinoic acid (RA) and/or cAMP elevating agents have been shown to promote differentiation of embryonal carcinoma (EC) cells. In this report we examine the response of different EC cell lines to different combinations of RA and cAMP elevating reagents. The degree of differentiation was measured via mRNA levels of stage specific markers: Endo A, collagen IV and tissue plasminogen activator. We show that complete differentiation cannot be achieved with cAMP elevating agents alone. Furthermore, the results obtained with each cell line are quite distinct and the degree of differentiation differs with the type of cAMP elevating agent used and the marker examined.
