Open Access

Hypermethylation of promoter regions of the APC1A and p16INK4a genes in relation to prognosis and tumor characteristics in cervical cancer patients

  • Authors:
    • Zarah M. Löf-Öhlin
    • Bengt Sorbe
    • Sten Wingren
    • Torbjörn K. Nilsson
  • View Affiliations

  • Published online on: June 14, 2011     https://doi.org/10.3892/ijo.2011.1078
  • Pages: 683-688
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Abstract

Hypermethylation of the O6-MGMT, p14ARF, p16INK4a, RASSF1A and APC1A genes are unfavourable prognostic markers in colorectal cancer (CRC). We hypothesized that they could be related to prognosis also in cervical cancer. Methylation was studied in DNA extracts from surgical specimens of cancer tissue by novel pyrosequencing methods. In 109 patients (90 squamous cell carcinomas, 19 adenocarcinomas), we found that hypermethylation of the APC1A gene promoter occurred in 8.3% of patients, and of p16INK4a in 1.8%. APC1A hypermethylation was significantly related to more advanced FIGO stage of the tumor (P=0.013), larger tumor diameter (P=0.049) and distant recurrence-free survival (P=0.0007), but not with locoregional recurrence rate, age, HPV status, DNA ploidy, tumor grade or malignancy grading score. We conclude that methylation of the APC1A promoter in cervical cancer, as diagnosed by pyrosequencing, is significantly related to major biological characteristics of the tumor, and may be a new predictor of poor prognosis in cervical cancer.

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September 2011
Volume 39 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Löf-Öhlin ZM, Sorbe B, Wingren S and Nilsson TK: Hypermethylation of promoter regions of the APC1A and p16INK4a genes in relation to prognosis and tumor characteristics in cervical cancer patients. Int J Oncol 39: 683-688, 2011.
APA
Löf-Öhlin, Z.M., Sorbe, B., Wingren, S., & Nilsson, T.K. (2011). Hypermethylation of promoter regions of the APC1A and p16INK4a genes in relation to prognosis and tumor characteristics in cervical cancer patients. International Journal of Oncology, 39, 683-688. https://doi.org/10.3892/ijo.2011.1078
MLA
Löf-Öhlin, Z. M., Sorbe, B., Wingren, S., Nilsson, T. K."Hypermethylation of promoter regions of the APC1A and p16INK4a genes in relation to prognosis and tumor characteristics in cervical cancer patients". International Journal of Oncology 39.3 (2011): 683-688.
Chicago
Löf-Öhlin, Z. M., Sorbe, B., Wingren, S., Nilsson, T. K."Hypermethylation of promoter regions of the APC1A and p16INK4a genes in relation to prognosis and tumor characteristics in cervical cancer patients". International Journal of Oncology 39, no. 3 (2011): 683-688. https://doi.org/10.3892/ijo.2011.1078