HPV-16 E6 and E7 oncogene expression is downregulated as a result of Mdm2 knockdown

Diaz,Daniel; Santander,Mauricio  A. Salinas; Chavez,Jesús  A. Morlett

doi:10.3892/ijo.2012.1429

HPV-16 E6 and E7 oncogene expression is downregulated as a result of Mdm2 knockdown

Authors:
- Daniel Diaz
- Mauricio A. Salinas Santander
- Jesús A. Morlett Chavez
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Affiliations: Department of Clinic Analysis and Molecular Diagnostic, Faculty of Chemistry, Universidad Autónoma de Coahuila, Republica Oriente, CP 25280, Saltillo, Coahuila, Mexico, Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico
Published online on: April 10, 2012 https://doi.org/10.3892/ijo.2012.1429
Pages: 141-146

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Abstract

The carcinogenic potential of HPV infections is based on the integration and constitutive expression of the E6 and E7 genes which inhibit the p53 and Rb tumor suppressor proteins. In normal cells, Mdm2 regulates p53 in a negative feedback loop, and although Mdm2 is apparently functional in HPV-infected cells, E6 is the protein responsible for repressing p53 replacing Mdm2 function. The role of Mdm2 in HPV-positive cells is still elusive. In this study, Mdm2 was knocked down in an HPV-positive cervical cancer cell line; as a result we found downregulation of the expression of E6 and E7 and p53 upregulation.