PAX2 is an antiapoptotic molecule with deregulated expression in medulloblastoma

Burger,M. C.; Brucker,D.  P.; Baumgarten,P.; Ronellenfitsch,M. W.; Wanka,C.; Hasselblatt,M.; Eccles,M.  R.; Klingebiel,T.; Weller,M.; Rieger,J.; Mittelbronn,M.; Steinbach,J. P.

doi:10.3892/ijo.2012.1446

PAX2 is an antiapoptotic molecule with deregulated expression in medulloblastoma

Authors:
- M. C. Burger
- D. P. Brucker
- P. Baumgarten
- M. W. Ronellenfitsch
- C. Wanka
- M. Hasselblatt
- M. R. Eccles
- T. Klingebiel
- M. Weller
- J. Rieger
- M. Mittelbronn
- J. P. Steinbach
View Affiliations

Affiliations: Dr Senckenberg Institute of Neurooncology, Goethe-University Hospital Frankfurt, Frankfurt, Germany, Institute of Neurology (Edinger Institute), Goethe-University Hospital Frankfurt, Frankfurt, Germany, Institute of Neuropathology, University Hospital Münster, Münster, Germany, Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand, Klinik für Kinder- und Jugendmedizin III, Goethe-University Hospital Frankfurt, Frankfurt, Germany, Department of Neurology, University Hospital Zürich, Zürich, Switzerland
Published online on: April 25, 2012 https://doi.org/10.3892/ijo.2012.1446
Pages: 235-241

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

PAX2 is a paired box transcription factor possessing a fundamental role in the embryogenesis of hindbrain and urinary tract. PAX genes are proto-oncogenes, PAX2 expression may contribute to the pathogenesis of renal cell carcinoma. Because of the expression of PAX2 in the developing hindbrain and its essential role in cerebellar development, it has been hypothesized that PAX2 may also be involved in medulloblastoma tumorigenesis. We investigated the expression pattern of PAX2 and various genes of the neuronal lineage in medulloblastoma and glioma cell lines. We found high expression of PAX2 mRNA and PAX2 protein in medulloblastoma cells and some glioma cell lines independent of their neuronal lineage gene expression signature. Gene suppression of PAX2 decreased the expression of the PAX2 transcriptional target GDNF in Daoy cells and had a profound cytotoxic effect in vitro on Daoy medulloblastoma and T98G glioma cells. Expression of PAX2 was then assessed in two separate medulloblastoma tissue microarrays with a total of 61 patient samples by immunohistochemistry. PAX2 expression was detected in the majority of medulloblastoma samples and correlated with less differentiated histology. Therefore, PAX2 is a biomarker for a more aggressive medulloblastoma phenotype and may represent a novel therapeutic target.