Human colorectal CD24+ cancer stem cells are susceptible to epithelial-mesenchymal transition

Okano,Miho; Konno,Masamitsu; Kano,Yoshihiro; Kim,Hirotoshi; Kawamoto,Koichi; Ohkuma,Masahisa; Haraguchi,Naotsugu; Yokobori,Takehiko; Mimori,Koshi; Yamamoto,Hirofumi; Sekimoto,Mitsugu; Doki,Yuichiro; Mori,Masaki; Ishii,Hideshi

doi:10.3892/ijo.2014.2462

Human colorectal CD24+ cancer stem cells are susceptible to epithelial-mesenchymal transition

Authors:
- Miho Okano
- Masamitsu Konno
- Yoshihiro Kano
- Hirotoshi Kim
- Koichi Kawamoto
- Masahisa Ohkuma
- Naotsugu Haraguchi
- Takehiko Yokobori
- Koshi Mimori
- Hirofumi Yamamoto
- Mitsugu Sekimoto
- Yuichiro Doki
- Masaki Mori
- Hideshi Ishii
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Affiliations: Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan, Department of Frontier Science for Cancer and Chemotherapy, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan, Department of Surgery, Kyushu University Beppu Hospital, Tsurumihara 4546, Oita 874-0838, Japan
Published online on: May 23, 2014 https://doi.org/10.3892/ijo.2014.2462
Pages: 575-580

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Abstract

Conventional cancer chemotherapy preferentially destroys non-stem cancer cells within a tumor, and a subpopulation of cancer stem cells (CSCs) is more resistant and survives, leading to relapses and metastasis. Howeve, recent studies suggest that CD24 and susceptibility to epithelial-mesenchymal transition (EMT) can serve as markers of CSCs. We report that CD24+ cells are susceptible to induction of EMT, a phenotype important for cancer metastasis. We studied the responsiveness of CSC markers to TGF-?, an effective EMT inducer. The data on CD24 demonstrated that CD24+ cells are susceptible to EMT, a phenotype important for cancer metastasis in two colorectal cancer cell lines, the CaR-1 and CCK81. CD24+ cells expressed Notch 1 in response to exposure to TGF-? in culture and showed higher tumorigenic activity compared to controls. This evidence shows that CD24+ cells are susceptible to EMT induction and to cancer progression and is indicative of the candidacy of CD24 as a therapeutic target in CSC.

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