Open Access

Lidocaine induces protective autophagy in rat C6 glioma cell line

  • Authors:
    • Magdalena Izdebska
    • Marta Hałas-Wiśniewska
    • Wioletta Zielińska
    • Anna Klimaszewska-Wiśniewska
    • Dariusz Grzanka
    • Maciej Gagat
  • View Affiliations

  • Published online on: December 14, 2018     https://doi.org/10.3892/ijo.2018.4668
  • Pages: 1099-1111
  • Copyright: © Izdebska et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Malignant glioma is the most common type of brain cancer with poor prognosis. Surgical resection, chemotherapy and radiotherapy are the main therapeutic options; however, in addition to their insufficient efficacy, they are associated with the pain experienced by patients. To relieve pain, local anesthetics, such as lidocaine can be used. In the present study, the effects of lidocaine on the C6 rat glioma cell line were investigated. An MTT assay and Annexin V/propidium iodide analysis indicated the increase in the percentage of apoptotic and necrotic cells in response to lidocaine. Furthermore, light microscopy analysis on the ultrastructural level presented the occurrence of vacuole‑like structures associated with autophagy, which was supported by the analysis of autophagy markers (microtubule‑associated protein 1A/1B‑light chain 3, acridine orange and Beclin‑1). Additionally, reorganization of the cytoskeleton was observed following treatment with lidocaine, which serves an important role in the course of autophagy. To determine the nature of autophagy, an inhibitor, bafilomycin A1 was applied. This compound suppressed the fusion of autophagosomes with lysosomes and increased the percentage of apoptotic cells. These results demonstrated that lidocaine may induce cytoprotective autophagy and that manipulation of this process could be an alternative therapeutic strategy in the treatment of cancer.
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Journal Cover

March-2019
Volume 54 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Izdebska M, Hałas-Wiśniewska M, Zielińska W, Klimaszewska-Wiśniewska A, Grzanka D and Gagat M: Lidocaine induces protective autophagy in rat C6 glioma cell line. Int J Oncol 54: 1099-1111, 2019.
APA
Izdebska, M., Hałas-Wiśniewska, M., Zielińska, W., Klimaszewska-Wiśniewska, A., Grzanka, D., & Gagat, M. (2019). Lidocaine induces protective autophagy in rat C6 glioma cell line. International Journal of Oncology, 54, 1099-1111. https://doi.org/10.3892/ijo.2018.4668
MLA
Izdebska, M., Hałas-Wiśniewska, M., Zielińska, W., Klimaszewska-Wiśniewska, A., Grzanka, D., Gagat, M."Lidocaine induces protective autophagy in rat C6 glioma cell line". International Journal of Oncology 54.3 (2019): 1099-1111.
Chicago
Izdebska, M., Hałas-Wiśniewska, M., Zielińska, W., Klimaszewska-Wiśniewska, A., Grzanka, D., Gagat, M."Lidocaine induces protective autophagy in rat C6 glioma cell line". International Journal of Oncology 54, no. 3 (2019): 1099-1111. https://doi.org/10.3892/ijo.2018.4668
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