17β-estradiol-induced activation of ERK1/2 through endogenous androgen receptor-estradiol receptor α-Src complex in human prostate cells
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- Published online on: September 1, 2003 https://doi.org/10.3892/ijo.23.3.797
- Pages: 797-801
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Abstract
We examined the effect of estrogens on mitogen-activated protein kinase (MAPK) in EPN cells, a line of epithelial cells derived from human normal prostate. 17β-estradiol (E2) caused a rapid and transient activation of MAPK (ERK1/2) within 5 min. This effect was counteracted by the anti-estrogen ICI 182-780 and by MEK inhibitor PD098059. The activation of ERK1/2 through 17β-estradiol triggered simultaneous association of endogenous androgen receptor, estrogen receptor α and Src. In addition, E2 stimulated the proliferation of EPN cells, suggesting that the formation of the ternary complex and the consequent activation of ERKs are implicated in the mechanism regulating proliferation of epithelial prostate cells.