Similar immune responses and antitumor effects of murine dendritic cells loaded with either exogenous or endogenous-synthesized protein

  • Authors:
    • Roger Lacave
    • Carole Masurier
    • Martine Guigon
    • François M. Lemoine
    • David Klatzmann
    • Bruno M. Colombo
  • View Affiliations

  • Published online on: December 1, 2003     https://doi.org/10.3892/ijo.23.6.1747
  • Pages: 1747-1753
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Conceptually, dendritic cells (DCs) take up and process exogenous antigens that are presented on MHC (major histocompatibility complex) class II molecules to stimulate CD4+ T cells, and present endogenously-produced proteins to CD8+ T cells through an MHC class I-dependent pathway. In this study, we compared the antitumor effects generated in vivo after vaccinations with DCs either loaded with exogenous protein (DCs-exo) or presenting antigens derived from endogenous-synthesized protein (DCs-endo). We used the murine MC26SC31 colon carcinoma cell line expressing on the cell surface the human CD4 (hCD4) molecule as a model tumor-associated antigen (TAA). Bone marrow (BM)-derived DCs-endo were obtained from histocompatible transgenic mice constitutively expressing hCD4; BM-derived DCs-exo were obtained after in vitro loading DCs, from syngenic normal mice, with purified soluble hCD4 protein (shCD4). Altogether, our results show that intravenous (i.v.) administration of DCs-exo and DCs-endo can trigger similar cytotoxic and humoral protecting immune responses against a lethal tumor challenge. hCD4 antigen-specific T cell-mediated cytotoxic immune response was not accompanied by elevated INF-γ serum levels. This suggests, in the case of DCs-exo, a possible in vivo CTL cross-priming triggering a CD8+ T cell-mediated immune response in the absence of de novo antigen synthesis within the DCs.

Related Articles

Journal Cover

December 2003
Volume 23 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lacave R, Masurier C, Guigon M, Lemoine FM, Klatzmann D and Colombo BM: Similar immune responses and antitumor effects of murine dendritic cells loaded with either exogenous or endogenous-synthesized protein. Int J Oncol 23: 1747-1753, 2003.
APA
Lacave, R., Masurier, C., Guigon, M., Lemoine, F.M., Klatzmann, D., & Colombo, B.M. (2003). Similar immune responses and antitumor effects of murine dendritic cells loaded with either exogenous or endogenous-synthesized protein. International Journal of Oncology, 23, 1747-1753. https://doi.org/10.3892/ijo.23.6.1747
MLA
Lacave, R., Masurier, C., Guigon, M., Lemoine, F. M., Klatzmann, D., Colombo, B. M."Similar immune responses and antitumor effects of murine dendritic cells loaded with either exogenous or endogenous-synthesized protein". International Journal of Oncology 23.6 (2003): 1747-1753.
Chicago
Lacave, R., Masurier, C., Guigon, M., Lemoine, F. M., Klatzmann, D., Colombo, B. M."Similar immune responses and antitumor effects of murine dendritic cells loaded with either exogenous or endogenous-synthesized protein". International Journal of Oncology 23, no. 6 (2003): 1747-1753. https://doi.org/10.3892/ijo.23.6.1747