Comparison of extracellular matrix and apoptotic markers between benign lesions and carcinomas in human breast

  • Authors:
    • Fortunata Vasaturo
    • Elvira Sallusti
    • Angela Gradilone
    • Carolina Malacrino
    • Tiziana Nardo
    • Grazia Avagnano
    • Anna Maria Aglianò
    • Teresa Granato
    • Bruno De Vincenzi
    • Giuseppe Coppotelli
    • Antonella Marzullo
    • Giuseppe Soda
    • Lucilla Simonelli
    • Mauro Modesti
    • Susanna Scarpa
  • View Affiliations

  • Published online on: October 1, 2005     https://doi.org/10.3892/ijo.27.4.1005
  • Pages: 1005-1011
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Abstract

The expression of the extracellular matrix-related genes, such as fibronectin, laminin and tenascin C, and apoptosis-related genes, such as bax, bcl2 and survivin, was evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and by immunohistochemistry in normal breast tissue and benign and malignant breast tumors and then correlated to several clinical parameters: estrogen and progesterone receptors, Ki67, ErbB2, tumor size, lymph node status and grading. Seventy-three breast tissue samples were examined. After RNA extraction, an RT-PCR was performed to detect fibronectin, laminin, tenascin C, bax, bcl2 and survivin gene expression. Thirty-two samples were evaluated also by immunohistochemistry at the protein level to detect fibronectin, laminin, tenascin C, bax and survivin. We found a significant correlation (P=0.025) between fibronectin gene expression and lymph node status, and a significant negative correlation (P=0.049) between laminin gene expression and Ki67. In addition, we found a statistically significant increase in survivin transcription in malign tumors compared to fibroadenomas (P=0.024). The negative correlation between laminin transcription and Ki67 could suggest that laminin impacts negatively on tumor proliferation, and the positive correlation between fibronectin and lymph node status may lead to consider fibronectin as predictive of long distance metastasis.

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October 2005
Volume 27 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Vasaturo F, Sallusti E, Gradilone A, Malacrino C, Nardo T, Avagnano G, Aglianò AM, Granato T, De Vincenzi B, Coppotelli G, Coppotelli G, et al: Comparison of extracellular matrix and apoptotic markers between benign lesions and carcinomas in human breast. Int J Oncol 27: 1005-1011, 2005.
APA
Vasaturo, F., Sallusti, E., Gradilone, A., Malacrino, C., Nardo, T., Avagnano, G. ... Scarpa, S. (2005). Comparison of extracellular matrix and apoptotic markers between benign lesions and carcinomas in human breast. International Journal of Oncology, 27, 1005-1011. https://doi.org/10.3892/ijo.27.4.1005
MLA
Vasaturo, F., Sallusti, E., Gradilone, A., Malacrino, C., Nardo, T., Avagnano, G., Aglianò, A. M., Granato, T., De Vincenzi, B., Coppotelli, G., Marzullo, A., Soda, G., Simonelli, L., Modesti, M., Scarpa, S."Comparison of extracellular matrix and apoptotic markers between benign lesions and carcinomas in human breast". International Journal of Oncology 27.4 (2005): 1005-1011.
Chicago
Vasaturo, F., Sallusti, E., Gradilone, A., Malacrino, C., Nardo, T., Avagnano, G., Aglianò, A. M., Granato, T., De Vincenzi, B., Coppotelli, G., Marzullo, A., Soda, G., Simonelli, L., Modesti, M., Scarpa, S."Comparison of extracellular matrix and apoptotic markers between benign lesions and carcinomas in human breast". International Journal of Oncology 27, no. 4 (2005): 1005-1011. https://doi.org/10.3892/ijo.27.4.1005