Effect of sulforaphane on cell growth, G0/G1 phase cell progression and apoptosis in human bladder cancer T24 cells
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- Published online on: October 1, 2006 https://doi.org/10.3892/ijo.29.4.883
- Pages: 883-888
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Abstract
Isothiocyanates (ITCs) from cruciferous vegetables have been shown to be effective in blocking initiation as well as progression of a range of chemically-induced tumors in animal models. In this study, sulforaphane, the most extensively studied ITC, was found to suppress the growth of T24 bladder cancer cells in vitro in a dose-dependent manner. Sulforaphane inhibited the proliferation of T24 cells with IC50 values 26.9 and 15.9 µM following 24 and 48 h treatments. Sulforaphane (5-20 µM) induced early apoptosis and blocked cell cycle progression at G0/G1 phase which was associated with upregulation of cyclin-dependent kinase inhibitor p27 expression. These results support a role for sulforaphane as an effective agent in the chemoprevention of bladder cancer.
