Effects of Agelas oroides and Petrosia ficiformis crude extracts on human neuroblastoma cell survival

  • Authors:
    • Cristina Ferretti
    • Barbara Marengo
    • Chiara De Ciucis
    • Mariapaola Nitti
    • Maria Adelaide Pronzato
    • Umberto Maria Marinari
    • Roberto Pronzato
    • Renata Manconi
    • Cinzia Domenicotti
  • View Affiliations

  • Published online on: January 1, 2007     https://doi.org/10.3892/ijo.30.1.161
  • Pages: 161-169
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Abstract

Among marine sessile organisms, sponges (Porifera) are the major producers of bioactive secondary metabolites that defend them against predators and competitors and are used to interfere with the pathogenesis of many human diseases. Some of these biological active metabolites are able to influence cell survival and death, modifying the activity of several enzymes involved in these cellular processes. These natural compounds show a potential anticancer activity but the mechanism of this action is largely unknown. In this study, we investigated the effects of two Mediterranean sponges, Agelas oroides and Petrosia ficiformis on the viability of human neuroblastoma cells. Upon treatment with the methanolic extract of Petrosia ficiformis, a marked cytotoxic effect was observed at any concentration or time of exposure. In contrast, a time- and dose-dependent effect was monitored for Agelas oroides that induced the development of apoptotic features and ROS production in LAN5 cells. These events were suppressed by calpeptin or zVAD and by vitamin C suggesting that the cell death caused by Agelas oroides was calpain- and caspase-dependent and of oxidative nature. Comet assay showed that this methanolic extract was not able to produce a genotoxic effect. Future studies will be applied to investigate the effect of isolated bioactive compounds from crude extract of this sponge which are potentially useful for cancer therapeutics.

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January 2007
Volume 30 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ferretti C, Marengo B, De Ciucis C, Nitti M, Pronzato MA, Marinari UM, Pronzato R, Manconi R and Domenicotti C: Effects of Agelas oroides and Petrosia ficiformis crude extracts on human neuroblastoma cell survival. Int J Oncol 30: 161-169, 2007.
APA
Ferretti, C., Marengo, B., De Ciucis, C., Nitti, M., Pronzato, M.A., Marinari, U.M. ... Domenicotti, C. (2007). Effects of Agelas oroides and Petrosia ficiformis crude extracts on human neuroblastoma cell survival. International Journal of Oncology, 30, 161-169. https://doi.org/10.3892/ijo.30.1.161
MLA
Ferretti, C., Marengo, B., De Ciucis, C., Nitti, M., Pronzato, M. A., Marinari, U. M., Pronzato, R., Manconi, R., Domenicotti, C."Effects of Agelas oroides and Petrosia ficiformis crude extracts on human neuroblastoma cell survival". International Journal of Oncology 30.1 (2007): 161-169.
Chicago
Ferretti, C., Marengo, B., De Ciucis, C., Nitti, M., Pronzato, M. A., Marinari, U. M., Pronzato, R., Manconi, R., Domenicotti, C."Effects of Agelas oroides and Petrosia ficiformis crude extracts on human neuroblastoma cell survival". International Journal of Oncology 30, no. 1 (2007): 161-169. https://doi.org/10.3892/ijo.30.1.161