Quantification of MET and hepatocyte growth factor/scatter factor expression in colorectal adenomas, carcinomas and non-neoplastic epithelia by quantitative laser scanning microscopy

  • Authors:
    • Stephan E. Baldus
    • Eric J. Kort
    • Peter Schirmacher
    • Hans P. Dienes
    • James H. Resau
  • View Affiliations

  • Published online on: July 1, 2007     https://doi.org/10.3892/ijo.31.1.199
  • Pages: 199-204
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Abstract

Hepatocyte growth factor (HGF)-MET signalling in cancer biology has been well characterized in multiple organ systems. Numerous investigations have described an up-regulation of c-met mRNA in human colorectal adenomas and carcinomas. However, a quantitative immunohistochemical analysis of MET and HGF protein levels in tumor tissues has not been reported previously. Formalin-fixed and paraffin-embedded tissues from 41 colorectal adenomas and 49 colorectal carcinomas were characterized by immunofluorescent staining using HGF- and MET-specific antibodies. The immunoreactivity was evaluated by confocal laser scanning microscopy, computer-based image analysis and appropriate statistical tests. Normal colorectal mucosa, adenomas and carcinomas exhibited comparable levels of MET and HGF proteins. MET expression in carcinomas, although statistically not significant, demonstrated a tendency to correlate with the grade of differentiation. Correlations of MET and HGF with other clinico-pathological variables including the extent of the mucinous component and the pTNM stage were not observed. The ratio of HGF in carcinoma vs. non-neoplastic tissue was significantly different between high and low carcinoma stage. Alterations of absolute levels of MET and HGF protein during the colorectal adenoma-carcinoma sequence were not significant. The presumed role of MET-HGF interactions in large bowel carcinogenesis may therefore be a result of or depend upon other regulatory factors involved in MET-mediated signalling pathways.

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July 2007
Volume 31 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Baldus SE, Kort EJ, Schirmacher P, Dienes HP and Resau JH: Quantification of MET and hepatocyte growth factor/scatter factor expression in colorectal adenomas, carcinomas and non-neoplastic epithelia by quantitative laser scanning microscopy. Int J Oncol 31: 199-204, 2007.
APA
Baldus, S.E., Kort, E.J., Schirmacher, P., Dienes, H.P., & Resau, J.H. (2007). Quantification of MET and hepatocyte growth factor/scatter factor expression in colorectal adenomas, carcinomas and non-neoplastic epithelia by quantitative laser scanning microscopy. International Journal of Oncology, 31, 199-204. https://doi.org/10.3892/ijo.31.1.199
MLA
Baldus, S. E., Kort, E. J., Schirmacher, P., Dienes, H. P., Resau, J. H."Quantification of MET and hepatocyte growth factor/scatter factor expression in colorectal adenomas, carcinomas and non-neoplastic epithelia by quantitative laser scanning microscopy". International Journal of Oncology 31.1 (2007): 199-204.
Chicago
Baldus, S. E., Kort, E. J., Schirmacher, P., Dienes, H. P., Resau, J. H."Quantification of MET and hepatocyte growth factor/scatter factor expression in colorectal adenomas, carcinomas and non-neoplastic epithelia by quantitative laser scanning microscopy". International Journal of Oncology 31, no. 1 (2007): 199-204. https://doi.org/10.3892/ijo.31.1.199