TRANSFORMATION OF MURINE FIBROBLASTS BY UV-LIGHT AND TGF-BETA - ESTABLISHMENT OF AN AUTOCRINE TGF-BETA LOOP
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- Published online on: December 1, 1994 https://doi.org/10.3892/ijo.5.6.1341
- Pages: 1341-1346
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Abstract
Murine fibroblasts stably transformed by initiation with UV light and tumor promotion with TGF-beta form colonies in soft agar, exhibit a diffuse actin pattern and induce angiogenesis. They differ from their parental cells by their ability to release active TGF and to react to TGF-beta by increased colony formation. Antibodies directed against TGF-beta abrogate the transformed phenotype, indicating the action of an autocrine TGF-beta loop for the maintenance of the transformed state. However, TGF-beta which is responsible for the maintenance of the transformed state, proves to be a severe obstacle for the survival of transformed cells which are cocultivated with normal cells.
