IMMUNOHISTOCHEMICAL DETECTION OF THE LAMININ RECEPTOR POLYPEPTIDE, A PUTATIVE PRECURSOR OF 67-KDA-LAMININ RECEPTOR, IN HUMAN LUNG-CANCER
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- Published online on: July 1, 1995 https://doi.org/10.3892/ijo.7.1.123
- Pages: 123-126
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Abstract
Laminin receptor polypeptide was immunodetected in human lung cancer with a polyclonal antibody raised against a 20-mer peptide of the putative high-affinity laminin receptor of 67 kDa (Wewer et al: Cancer Res 47: 5691-5698, 1987). As a result, immunoreactivity was recognized specifically in cancer cells both in freshly-prepared cell samples and in paraffin-embedded tissue specimens. It was shown that immunodetection of the laminin receptor polypeptide with this antipeptide antibody could be applied to diagnostic use for lung cancer. The results also suggest that the laminin receptor polypeptide is not necessarily a membrane-associated protein and may function without further processing to the 67 kDa-laminin receptor.
